文献类型：期刊文献

中文题名：海洋放线菌抗菌活性菌株的筛选及南-148菌株的鉴定

英文题名：Screening of anti-bacterial marine-derived actinomycetes and identification of strain Nan-148

作者：岳蕾娜[1];温清玲[1];易润华[1];于莉[1];何红[1]

机构：[1]广东海洋大学农学院生物技术系,广东湛江524088

年份：2010

卷号：41

期号：2

起止页码：126

中文期刊名：广西农业科学

外文期刊名：Guangxi Agricultural Sciences

收录：CSTPCD、、CSCD_E2011_2012、CSCD

基金：湛江市科技攻关项目[湛财企(2004)98];广东海洋大学团队项目(0412111)

语种：中文

中文关键词：海洋放线菌;南-148菌株;鉴定;弗氏链霉菌

外文关键词：marine-derived aetinomyeetes; strain Nan-148; identification; Streptomyces fradiae

中文摘要：以桉树青枯病菌和金黄色葡萄球菌为指示菌,对从广东湛江近海海泥中分离得到的135株放线菌的抗菌活性进行测定,并对南-148菌株进行形态观察和生理生化特性鉴定。结果表明,135株海洋放线菌中有15株对桉树青枯病菌有抑制作用,24株对金黄色葡萄球菌有抑制作用,12株对两种病菌均有抑制作用,其中南-148菌株的抑菌效果最好,对桉树青枯病菌的抑菌圈直径为20.0mm,对金黄色葡萄球菌的抑菌圈直径为37.3mm;对南-148菌株进行了鉴定,初步确定南-148菌株为弗氏链霉菌(Streptomyces fradiae)。

外文摘要：The present study was conducted to determine the antibacterial activities of 135 marine-derived aetinomycetes isolated from sea mud of offshore sea area in Zhanjiang City, Guangdong Province, and to observe the morphological characteristics and identification of the physiological and biochemical characteristics of strain Nan-148. The Ralstonia solanacearum in Eucalyptus and Staphylococcus aureus were used as indicator strains. The results indicated that among 135 marine-derived actinomycetes, 15 isolates showed antibacterial effects against Ralstonia solanacearum, 24 isolates against Staphflococcus aurous, 12 isolates against both of them. Out of all, the strain Nan-148 showed the best antibacterial properties, and the diameter of inhibition zone to Staphflococcus aureus and Ralstonia solanacearum was found up to 37.3 and 20.0 mm, respectively. After culturing the bacteria and study of their physiological and biochemical charaeteristies, it was found that the strain Nan-148 was Streptomyces fradiae.