文献类型：期刊文献

英文题名：Molecular characterization and expression of CD2BP2 in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus

作者：Gan, Zhen[1,2,3];Wang, Bei[1,2,3];Lu, Yishan[1,2,3];Cai, Shuanghu[1,2,3];Cai, Jia[1,2,3];Jian, JiChang[1,2,3];Wu, Zaohe[2,3,4]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524025, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Anim, Zhanjiang 524025, Peoples R China;[4]Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

年份：2014

卷号：548

期号：1

起止页码：126

外文期刊名：GENE

收录：SCI-EXPANDED(收录号：WOS:000340329100018)、、Scopus(收录号：2-s2.0-84904718820)、WOS

基金：We thank all the laboratory members for their critical reviews and comments on this manuscript. We are especially grateful for the critical comments from the anonymous reviewers. This work was supported by research grants (No.31302226, No. 2012B020308009) from the National Natural Science Foundation of China and Science & Technology Planning Project of Guangdong Province of China.

语种：英文

外文关键词：Oreochromis niloticus; CD2BP2; RACE; Fluorescent quantitative real-time PCR; Streptococcus agalactiae

外文摘要：CD2BP2 (CD2 cytoplasmic tail binding protein 2), one of several proteins interacting with the cytoplasmic tail of CD2, plays a crucial role in CD2-triggered T cell activation and nuclear splicing. The studies on CD2BP2 have tended to be confined to a few mammals, and little information is available to date regarding fish CD2BP2. In this paper, a CD2BP2 gene (On-CD2BP2) was cloned from Nile tilapia, Oreochromis niloticus. Sequence analysis showed that the full length of On-CD2BP2 cDNA was 1429 bp, containing a 5'untranslated region (UTR) of 111 bp, a 3'-UTR of 193 bp and an open reading frame of 1125 bp which is encoding 374 amino acids. Two important structural features, a GYF domain and a consensus motif GPFXXXXMXXWXXXGYF were detected in the deduced amino acid sequence of On-CD2BP2, and the deduced genomic structure of On-CD2BP2 was similar to the known CD2BP2. The mRNA expression of On-CD2BP2 in various tissues of Nile tilapia was analyzed by fluorescent quantitative real-time PCR. In healthy Nile tilapia, the On-CD2BP2 transcripts were mainly detected in the head kidney and spleen. While vaccinated with inactivated Streptococcus agalactiae, the On-CD2BP2 mRNA expression was significantly up-regulated in the head kidney, spleen and brain 48 h post immunization. Moreover, there was a clear time-dependent expression pattern of On-CD2BP2 after immunization and the expression reached the highest level at 24 h in the brain and 48 h in the head kidney and spleen. This is the first report of proving the presence of a CD2BP2 ortholog in fish, and investigating its tissue distribution and expression profile in response to bacterial stimulus. These findings indicated that On-CD2BP2 may play an important role in the immune response to bacteria in Nile tilapia. (C) 2014 Elsevier B.V. All rights reserved.