文献类型：期刊文献

中文题名：美国红鱼血细胞观察

英文题名：A hematological study and observation on development of blood cells in American red fish(Sciaenops ocellatus)

作者：陈刚[1];周晖[1];叶富良[1];吴灶和[1]

机构：[1]广东海洋大学水产学院

年份：2006

卷号：25

期号：2

起止页码：59

中文期刊名：热带海洋学报

外文期刊名：Journal of Tropical Oceanography

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：广东省海洋与渔业局重大科技兴海项目(A200099A01)

语种：中文

中文关键词：美国红鱼;Sciaenops;ocellatus;血细胞发生;血液指标

外文关键词：Sciaenops ocellatus ; hematological index; development of blood cell

中文摘要：测定了美国红鱼(学名眼斑拟石首鱼Sciaenops ocellatus)的血液指标,红细胞密度为(1.95±0.37)×106个.mm-3,白细胞密度为(2.08±0.43)×104个.mm-3;血红蛋白含量为(6.65±1.06)g.L-1,红细胞渗透脆性为(0.40±0.06)g%,沉降速率为(1.15±0.10)mm.h-1。对美国红鱼外周血液进行涂片观察,在涂片上可区分出红细胞以及6种白细胞:血栓细胞、淋巴细胞、单核细胞、嗜酸性粒细胞、嗜中性粒细胞和嗜碱性粒细胞;它们在总的白细胞中所占比例分别为(68.42±11.62)%、(14.50±9.25)%、(1.46±1.80)%、(13.01±7.73)%和(2.6±3.76)%,嗜碱性粒细胞的含量极少,不到1%;在涂片上还发现了较多未成熟的红细胞。对肝脏、脾脏、头肾和肾脏等4种造血组织中血细胞的发生过程进行了观察和测定。观察发现,眼斑拟石首鱼的头肾和肾脏可以产生各种类型的血细胞,是主要的造血器官;脾脏是产生淋巴细胞的重要场所;而肝脏则在眼斑拟石首鱼粒细胞发生中扮演重要角色。

外文摘要：Hematological indices of American red fish （Sciaenops ocellatus） were investigated. The erythrocyte and leukocyte counts of American red fish were（1. 95±0.37） ×10^6 ind · mm^-3and （2.08± 0.43） × 10^4 ind · mm^-3 respectively, the hemoglobin content was（6.65±1.06）g ·L^-1, the erythrocyte osmotic fragility was 0.40±0.06g%, and the erythrocyte sedimentation rate was（1.15±0.10） mm · h^-1. On the stained smears of peripheral blood, erythrocytes, thrombocytes, lymphocytes, monocytes, ensinophilic granulocytes, neutrophils, and basophilic granulocytes were recognized. The percentages of thrombocytes, lymphocytes, monocytes, ensinophilic granulocytes, and neutrophils in total leucocytes were（68.42±11. 62）%, （14.50±9.25）%, （1.46±1.80）%, （13.01±7.73）% and（2.61±3.76）%, respectively. Some immature blood cells could also be observed on the smears. The development of the blood cells in liver, spleen, pronephros and kidney was studied. The results showed that the blood cells of American red fish develops mainly in pronephros and kidney; spleen is an important tissue for the development of lymphocytes and works as an assistant for the generation of monocytes and granulocytes, while liver is important for the development of granulocytes. The development of thrombocyte had not been observed in these four tissues.