文献类型：期刊文献

英文题名：Isolation and cDNA cloning of a novel red colour-related pigment-binding protein derived from the shell of the shrimp, Litopenaeus vannamei

作者：Pan, Chuang[1]; Ishizaki, Shoichiro[1]; Nagashima, Yuji[1]; Gao, Jialong[2]; Watabe, Shugo[3]

机构：[1] Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan; [2] College of Food Science and Technology, Guangdong Ocean University, Haida Road 1, Mazhang, Zhanjiang, Guangdong, 524088, China; [3] School of Marine Biosciences, Kitasato University, Sagamihara, 252-0373, Japan

年份：2018

卷号：241

起止页码：104

外文期刊名：Food Chemistry

收录：EI(收录号：20173504100970)

语种：英文

外文关键词：Proteins - Acetone - Pigments - Shells (structures) - Cloning - Amino acids

外文摘要：Pigment-binding proteins play important roles in crustacean shell colour change. In this study, a red colour-related pigment-binding protein, designated LvPBP75, was purified from the shell of Litopenaeus vannamei. HPLC and PAGE analysis showed that LvPBP75 was a homogeneous monomer with molecular mass of 75 kDa. Peptide mass fingerprint analysis revealed that LvPBP75 belonged to hemocyanin, and the released pigment from heated LvPBP75 showed a λmax at 481 nm in acetone. The significant red-colour change temperatures were detected at 30 and 80 °C, respectively. Based on the determined amino acid fragments, a full-length cDNA of LvPBP75 was cloned and sequenced. The ORF encodes a protein of 662 amino acids having 80% identity with penaeidae hemocyanin. These results strongly suggest a novel function of hemocyanin, namely binding with pigment, and its involvement in L. vannamei shell colour change. ? 2017 Elsevier Ltd