文献类型：期刊文献

中文题名：黄秋葵子叶离体再生体系的建立

英文题名：ESTABLISHMENT OF A SYSTEM FOR IN VITRO REGENERATION FOR ABELEMOSCHUS ESCULENTUS L.

作者：丰锋[1]

机构：[1]湛江海洋大学农学院,广东湛江524088

年份：2005

卷号：27

期号：1

起止页码：81

中文期刊名：西南农业大学学报（自然科学版）

外文期刊名：Journal of Southwest Agricultural University

收录：CSTPCD、、北大核心2004、CSCD_E2011_2012、北大核心、CSCD

语种：中文

中文关键词：黄秋葵;带子叶和部分下胚轴的顶芽;组织培养;再生体系

外文关键词：Abelemoxhlu esculentus L. ; apex with the cotyledons and part of the hypocotyls; tissue culture; regeneration system

中文摘要：以带子叶和部分下胚轴的顶芽为外植体,建立再生体系。结果表明,下胚轴长度为2 mm时有利于不定芽的形 成,7-10 d苗龄的外植体最易诱导出不定芽,芽分化和生长的最适温度为25±1℃。6-BA<1 mg／L不定芽难以发 生,6-BA 6+NAA 0.1有利于不定芽形成;CA3 2.0可以提高不定芽的诱导率。

外文摘要：Shoot apices with the cotyledons and part of the hypocotyls were used as explants in tissue culture for Abelemoschus esculentus L. regeneration. Hypocotyls 2 mm long were favorable for adventitious bud formation. The optimum temperature for bud differentiation and growth was 25±1℃. Adventitious buds were most readily induced from the explants originating from 7-10 day old seedlings. No adventitious buds were induced on media with 6 - BA at a concentration of < 1 mg/L. the optimal medium for adventitious bud regeneration in this experiment was MS + 6 - BA 6 mg/L + NAA 0.1 mg/L. Addition of GA3 at 2.0 mg/L to the medium raised the induction frequency of adventitious buds.