文献类型：期刊文献

英文题名：Integrated analysis neurimmiRs of tilapia (Oreochromis niloticus) involved in immune response to Streptococcus agalactiae, a pathogen causing meningoencephalitis in teleosts

作者：Wang, Bei[1];Gan, Zhen[1,2];Wang, Zhongliang[1];Yu, Dapeng[1];Lin, Ziwei[1];Lu, Yishan[1];Wu, Zaohe[1];Jian, Jichang[1]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Guangdong Higher Educ Inst,Key Lab Control Dis Aq, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[2]Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

年份：2017

卷号：61

起止页码：44

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000394200400006)、、WOS

基金：The authors wish to thank all the laboratory member suggestion on this manuscript. This work supported by National Natural Science Foundation of China (Grant No. 31302226), Science & Technology Planning Project of Guangdong Province of China (Grant No. 2013B020307014) and Project of Enhancing School with Innovation (Grant No. 201602121). We are especially grateful for the critical comments from the anonymous reviewers. The authors also thank Genedenovo Biothchnology Co., Ltd. (Guangzhou, China) for miRNAs sequence and assisting in the revision of this manuscript.

语种：英文

外文关键词：Oreochromis niloticus; MicroRNA; Streptococcus agalactiae; Neuroinflammation; Immune response

外文摘要：MicroRNAs (miRNAs) are a class of noncoding RNA molecules and play important roles in a wide spectrum of biological processes, including in immune response. Recent years have witnessed considerable amount of research interest in studies on miRNA-mediated modulation gene function during neuroinflammation. Here, we evaluated Streptococcus agalactiae infected tilapia (Oreochromis niloticus) brain for the expression profile of miRNAs, potential functions and their correlation with genes involved in inflammatory pathways. A total of 1981 miRNAs were identified, including in 486 miRNAs which have homologues in the currently available databases and 1945 novel miRNAs. The expression levels of 547 miRNAs were significantly altered at 6 h-48 h post-bacterial infection, and these miRNAs were therefore classified as differentially expressed tilapia miRNAs. Real-time PCR were implemented for 14 miRNAs co-expressed in five samples, and agreement was confirmed between the high-throughput sequencing and real-time PCR data. For the 486 differentially expressed miRNAs target 41,820 genes. GO and KEGG enrichment analysis revealed that some target genes of miRNAs were grouped mainly into the categories of apoptotic, signal pathway and immune response. This is the first report of comprehensive identification of teleost miRNAs being differentially regulated in brain in normal conditions relating to bacterial infection. (C) 2016 Elsevier Ltd. All rights reserved.