文献类型：期刊文献

英文题名：Modulation of Intestinal Barrier, Inflammatory Response, and Gut Microbiota by Pediococcus pentosaceus zy-B Alleviates Vibrio parahaemolyticus Infection in C57BL/6J Mice

作者：Wang, Rundong[1,2,3];Deng, Yijia[1,2];Zhang, Yuhao[1];Li, Xuepeng[2];Sun, Lijun[4];Deng, Qi[4];Liu, Ying[4];Gooneratne, Ravi[5];Li, Jianrong[1,2]

机构：[1]Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China;[2]Bohai Univ, Coll Food Sci & Engn, Jinzhou 121013, Peoples R China;[3]Lingnan Normal Univ, Coll Food Sci & Engn, Zhanjiang 524048, Peoples R China;[4]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[5]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand

年份：2022

卷号：70

期号：6

起止页码：1865

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000756703000011)、、EI(收录号：20220711632976)、Scopus(收录号：2-s2.0-85124478346)、WOS

基金：This work was supported by the National Key R&D Program of China (2019YFD0901702) , the young innovative program of Guangdong provincial department of education (2019KQNCX072) , and the characteristic innovation project of Guangdong provincial department of education (2020KTSCX071) .

语种：英文

外文关键词：Pediococcus pentosaceus zy-B; protective effect; Vibrio parahaemolyticus; intestinal homeostasis; gut microbiota

外文摘要：Modulation of the intestinal barrier, inflammation, and gut microbiota by Pediococcus pentosaceus zy-B (zy-B) in Vibrio parahaemolyticus (Vp)-infected C57BL/6J mice was studied. Mice intragastrically pretreated with 108 colony-forming units (CFU) zy-B significantly alleviated Vp infection as evidenced by maintaining body weight and reduced disease activity index score and intestine ratio. In addition, zy-B reduced the Vp load in the ileum and cecum, significantly reduced the load in the colon, prevented colonic atrophy, and strengthened mucosal integrity. Mechanistically, zy-B ameliorated intestinal barrier dysfunction by upregulating tight junction protein expression, which in turn reduced the lipopolysaccharide, D-lactic acid (D-LA), and diamine oxidase concentrations and downregulated the cannabinoid receptor 1 (CB1) and CB2 mRNA expressions. Moreover, zy-B systemically reduced inflammation by decreasing interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha levels, and increased interleukin-10 (IL-10), immunoglobulin M (IgM), and immunoglobulin G (IgG) levels in the colon and serum. Furthermore, zy-B markedly altered the gut microbiota composition by enriching Bifidobacterium, Akkermansia, and Lactobacillus in the colon. Overall, zy-B appears to act as a probiotic to alleviate Vp infection by protecting the intestinal barrier, reducing inflammation, and promoting the growth of "beneficial" gut microbiota.