文献类型：期刊文献

中文题名：文蛤蛋白质组成分析及其分子量分布研究

英文题名：Protein composition analysis and molecular weight distribution of Meretrix lusoria

作者：唐小艳[1];郑惠娜[1];章超桦[1];郝记明[1];张静[1]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东普通高等学校水产品深加工重点实验室,国家贝类加工技术研发分中心(湛江),广东湛江524088

年份：2015

卷号：36

期号：22

起止页码：362

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、北大核心2014、北大核心、CSCD_E2015_2016、CSCD

基金：国家现代农业产业技术体系(CARS-48-07B);广东省高校重大科研项目培育计划(GDOU2013050245);广东省高等学校优秀青年教师培养计划(Yq2014005);水产蛋白改性技术研究(2011A020102005)

语种：中文

中文关键词：文蛤;蛋白质分离;氨基酸组成;SDS-PAGE;DSC

外文关键词：Meretrix lusoria; protein separation; amino acid composition; SDS-PAGE; DSC;

中文摘要：为合理利用文蛤蛋白质资源,本文对其进行了蛋白质组分分离,得到了水溶性蛋白、盐溶性蛋白、不溶性蛋白及非蛋白氮4个组分,并分别对其中3个组分进行了一般成分、氨基酸组成、SDS-PAGE电泳及热稳定性分析。结果表明：新鲜文蛤含水量为84.9%、粗蛋白质含量为10.65%、灰分含量为1.20%、总糖含量为1.90%、粗脂肪含量为0.31%;4个蛋白组分占总蛋白含量分别为：盐溶性蛋白32.94%,水溶性蛋白44.69%,不溶性蛋白16.49%,非蛋白氮0.81%;文蛤蛋白氨基酸种类齐全,必需氨基酸比列均衡,必需氨基酸和呈味氨基酸含量高;SDS-PAGE电泳显示,文蛤蛋白质分子量分布广泛,但各组分之间存在一定差异性。盐溶性蛋白的分子量主要分布在200~66.4 ku和44.3~29.0 ku之间;水溶性蛋白的分子量主要集中在66.4~25 ku之间;不溶性蛋白主要集中在200~44.3 ku。DSC曲线显示：文蛤蛋白的变性温度在49.5℃,分离得到的盐溶性蛋白组分、水溶性蛋白组分及不溶性蛋白组分的热变性温度依次为：41.7、38.8和43.2℃。

外文摘要：In order to make full use of protein,the Meretrix lusoria protein was separated in this paper,and four components of protein：the water-soluble protein,the salt-soluble protein,insoluble protein and non-protein nitrogen were obtained,and the general composition,the consist of amino acid,the SDS-PAGE electrophoresis and the thermal stability of the three protein were analyzed respectively. The results showed that the moisture,crude protein,ash content,total sugar and crude fat of fresh Meretrix lusoria were 84.9%,10.65%,1.20%,1.90%,0.31%,respectively,among the raw protein,the salt-soluble protein,water-soluble protein,insoluble protein and non-protein nitrogen were accounted for 32.94%,44.69%,16.49%,0.81%,respectively. There were various of amino acids in Meretrix lusoria,and the ratio of essential amino acids were appropriate,the contents of essential amino acid and flavor amino acid were high. The results of SDS-PAGE showed that the protein molecular weight of Meretrix lusoria was widespread,but there were some differences among the components：the weight of salt-soluble protein molecular was mainly distributed in 200~66.4 ku and 44.3~29.0 ku,the weight of water-soluble protein molecular was mainly concentrated in 66.4~25 ku,the weight of insoluble protein was mainly concentrated in the 200~44.3 ku. The DSC curves showed that the denaturation temperature of Meretrix lusoria protein was 49.5 ℃,the denaturation temperature of salt-soluble protein,water-soluble and insoluble protein were 41.7,38.8 and 43.2 ℃,respectively.