文献类型：期刊文献

中文题名：马氏珠母贝近交与杂交家系的遗传结构比较

英文题名：Genetic Structure of Inbred and Hybrid Families of Pearl Oyster Pinctada martensii

作者：张嘉丽[1];罗燕秋[1];黄怡[1];钟雅诗[1];刘青云[1];李俊辉[1];邓岳文[1]

机构：[1]广东海洋大学水产学院,湛江524025

年份：2015

卷号：34

期号：4

起止页码：723

中文期刊名：基因组学与应用生物学

外文期刊名：Genomics and Applied Biology

收录：CSTPCD、、北大核心2014、CSCD2015_2016、北大核心、CSCD

基金：广东海洋大学创新课题"基于RNA-seq技术分析马氏珠母贝近交家系与杂交家系的遗传差异"(CXXL2014017);广东省海洋与渔业局科技专项"马氏珠母贝选系育珠性状测试与示范养殖"(A201308A10)共同资助

语种：中文

中文关键词：马氏珠母贝;近交家系;杂交家系;遗传结构;SSR标记

外文关键词：Pinctada martensii,Inbred families,Hybrid families,Genetic diversity,SSR Marker

中文摘要：分别从马氏珠母贝两个全同胞家系(A和B)选取亲本,按照因子设计构建如下4个家系:1号家系,A1♀×A2♂;2号家系,A1♀×B1♂;3号家系,B2♀×A2♂;4号家系,B1♀×B2♂。利用本实验室开发的13对SSR引物检测了4个家系的遗传多样性指标与遗传分化。结果表明1号、2号、3号和4号家系的平均观察杂合度分别为0.506 4、0.560 9、0.569 2和0.506 4。4个家系的遗传分化系数(Fst)为0.104 9。本研究结果表明,经过一代全同胞交配后,近交家系遗传多样性指标低于杂交家系。

外文摘要：Four families of pearl oyster Pinctada martensii were established by a two-factor design, with the breeders were sampled from two full-sib families(A and B). The four families were designated as follows: F1, A1♀×A2♂; F2, A1♀×B1♂; F3, B2♀×A2♂; F4, B2♀×B1♂. Genetic structure of the four families was analyzed by using 13 SSR primers. The results showed that there existed genetic differentiation among the four families(Fst=0.104 9). The average observed heterozygosity values of four families ranged from 0.506 4 to 0.569 2. Genetic diversity values of inbred families were lower relative to those of the hybrid families. The present results suggest that there should be a level of inbreeding after a generation of full-sib mating in pear oyster Pinctada martensii.