文献类型：期刊文献

英文题名：Genome and transcriptome analyses providing insight into the immune response of pearl oysters after allograft and xenograft transplantations

作者：Jiao, Yu[1];Yang, Shuai[1];Cao, Yanfei[1];Zheng, Zhe[1];Deng, Yuewen[1];Wang, Qingheng[1];Huang, Ronglian[1];Du, Xiaodong[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524025, Peoples R China

年份：2019

卷号：90

起止页码：109

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000471085000012)、、Scopus(收录号：2-s2.0-85065137476)、WOS

基金：The studies were financially supported by grants of the National Natural Science Foundation of China (grant numbers 31672626), the Guangdong Marine and Fishery Bureau (grant numbers Z2015004), Graduate education innovation program of Guangdong Ocean University (grant numbers 201839).

语种：英文

外文关键词：Pearl formation; Xenograft transplantation; Transcriptome; Genomic analysis; Pearl oyster

外文摘要：The immune response after allograft or xenograft transplantation in the pearl oyster is a major factor that cause its nucleus rejection and death. To determine the mechanism underlying the immune response after allograft and xenograft transplantations in the pearl oyster Pinctada fucata martensii, we constructed two sets of transcriptomes of hemocytes at different times (6 and 12 h; 1, 3, 6, 12, and 30 d) after allograft and xenograft transplantations, in which the xenografted mantle tissue was from Pinctada maxima. The transcriptomic analysis reveals many genes are involved in the immune response to transplantation, such as transient receptor potential cation channel (TRP), calmodulin (CaM), DNA replication-related genes, and sugar and lipid metabolism-related genes. The expression of these identified genes was higher in the host pearl oyster transplanted with xenograft than that by allograft. The histological analysis of the pearl sac also confirmed that many hemocytes were still gathered around the transplanted nucleus, and no pearl sac was formed in the host pearl oysters at 30 d after xenograft transplantation. The genomic analysis indicated that pearl oysters evolved many copies of genes, such as TRP, CaM, and GST, to sense and cope with the immune response after transplantation. "Ribosome" and "Cytosolic DNA-sensing pathway" were specifically induced in the xenograft group, whereas "Notch signaling pathway" specifically responded to the allograft transplantation. These results can improve our understanding of the mechanism underlying the immune response of pearl oysters after allograft and xenograft transplantations.