文献类型：期刊文献

英文题名：Structure of a laminarin-type β-(1→3)-glucan from brown algae Sargassum henslowianum and its potential on regulating gut microbiota

作者：Cui, Yongsheng[1];Zhu, Lixia[2,6];Li, Yixuan[1,3];Jiang, Siliang[1,3];Sun, Qili[1,3];Xie, Enyi[7];Chen, Hubiao[2];Zhao, Zhongzhen[2];Qiao, Wei[1];Xu, Jun[2,4,5];Dong, Caixia[1]

机构：[1]Tianjin Med Univ, Sch Pharm, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China;[2]Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Peoples R China;[3]Jiamusi Univ, Dept Pharmacognosy, Coll Pharm, Jiamusi 154007, Peoples R China;[4]China Acad Chinese Med Sci, Jiangsu Prov Acad Tradit Chinese Med, Dept Metab, Nanjing 210028, Peoples R China;[5]China Acad Chinese Med Sci, Jiangsu Branch, Nanjing 210028, Peoples R China;[6]Sun Yat Sen Univ, Affiliated Hosp 8, Shenzhen 518000, Peoples R China;[7]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China

年份：2021

卷号：255

外文期刊名：CARBOHYDRATE POLYMERS

收录：SCI-EXPANDED(收录号：WOS:000608727400002)、、EI(收录号：20204709510188)、Scopus(收录号：2-s2.0-85096194398)、WOS

基金：This work was supported by the National Natural Science Foundation of China (81803682 and 81872975), Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis (CTD2018-03), and Hong Kong General Research Fund (12100219 and 12103919).

语种：英文

外文关键词：Sargassum henslowianum; beta-(1 -> 3)-Glucan; In vitro fermentation; Gut microbiota; Short-chain fatty acids

外文摘要：A homogeneous polysaccharide named SHNP with apparent molecular weight of 8.4 kDa was purified from brown algae Sargassum henslowianum using ethanol precipitation, ion-exchange chromatography, and gel-filtration column chromatography. Structural analyses reveal that SHNP is completely composed of glucose, and its backbone consists of beta-D-(1 -> 3)-Glcp with side chains comprising t-beta-D Glcp attached at the O-6 position. Thus, SHNP is a laminarin-type polysaccharide. In vitro fermentation test results showed that SHNP was digested by gut microbiota; the pH value in the fecal culture of SHNP was significantly decreased; and total short-chain fatty acids, acetic, propionic and n-butyric acids were significantly increased. Furthermore, SHNP regulated the intestinal microbiota composition by stimulating the growth of species belonging to Enterobacteriaceae while depleting Haemophilus parainfluenzae and Gemmiger formicilis. Taken together, these results indicate that SHNP has the potential for regulating gut micmbiota, but its specific role in the regulation requires to be further investigated.