文献类型：期刊文献

英文题名：Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus)

作者：Li, Pengfei[1,2];Zhou, Lingli[1,2];Ni, Songwei[1,2];Xu, Meng[5];Yu, Yepin[1,2];Cai, Jia[3,4];Wei, Shina[1];Qin, Qiwei[1]

机构：[1]Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China;[2]Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China;[3]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[5]Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China

年份：2016

卷号：52

期号：4

起止页码：410

外文期刊名：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

收录：SCI-EXPANDED(收录号：WOS:000374349800003)、、WOS

基金：This study was supported by the National Basic Research Program of China (973) (2012CB114402) and grants from the National Natural Science Foundation of China (31330082) and grants from Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences (LMB141005).

语种：英文

外文关键词：Trachinotus ovatus; Brain cell line; Transfection efficiency; Virus susceptibility

外文摘要：Golden pompano is a commercially important marine fish that is widely cultured in China, Japan, and Southeast Asian countries but has been seriously threatened by pathogen. A novel cell line (TOGB) derived from the brain of golden pompano Trachinotus ovatus was established and characterized in this study. TOGB cell line showed high virus susceptibility, especially grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). As one of the most devastating viruses in marine fish aquaculture, nervous necrosis virus (NNV) causes high mortality rates exceeding 95% in severe outbreaks. Then, TOGB cell line was a useful tool for propagating viruses and provides a potentially valuable resource for the study of viral pathogenesis, the development of antiviral strategies. The TOGB cell lines showed potential application in environmental monitoring. The extracellular products from Vibrio anguillarum and Vibrio alginolyticus demonstrated cytotoxic effects in TOGB cells. TOGB cells grew most rapidly at 28A degrees C, with an optimal concentration of 10% fetal bovine serum in L-15 medium. TOGB cells were diploid (2N = 54). The transfection efficiencies of TOGB cells were 8.6% at the 15th passage and 64.8% at the 45th passage, indicating that the cells are suitable for foreign gene expression.