文献类型：期刊文献

英文题名：A neutral polysaccharide with a triple helix structure from ginger: Characterization and immunomodulatory activity

作者：Yang, Xiaolong[1];Wei, Siqing[2];Lu, Xiaoming[1];Qiao, Xuguang[1];Simal-Gandara, Jesus[3];Capanoglu, Esra[4];Wozniak, Lukasz[5];Zou, Liang[6];Cao, Hui[7];Xiao, Jianbo[3,7,8];Tang, Xiaozhen[1];Li, Ningyang[1]

机构：[1]Shandong Agr Univ, Coll Food Sci & Engn, Key Lab Food Proc Technol & Qual Control Shandong, Tai An, Shandong, Peoples R China;[2]Shandong Foreign Trade Vocat Coll, Tai An 271018, Shandong, Peoples R China;[3]Univ Vigo, Dept Analyt Chem & Food Sci, Nutr & Bromatol Grp, Fac Food Sci & Technol, Ourense Campus, E-32004 Orense, Spain;[4]Istanbul Tech Univ, Fac Chem & Met Engn, Dept Food Engn, TR-34469 Istanbul, Turkey;[5]Inst Agr & Food Biotechnol, Dept Fruit & Vegetable Prod Technol, 36 Rakowiecka St, PL-02532 Warsaw, Poland;[6]Chengdu Univ, Key Lab Coarse Cereal Proc, Minist Agr & Rural Affairs, Chengdu 610106, Peoples R China;[7]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[8]Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Jiangsu, Peoples R China

年份：2021

卷号：350

外文期刊名：FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000632284300009)、、EI(收录号：20210809953408)、Scopus(收录号：2-s2.0-85100909985)、WOS

基金：The project was supported by Major Agricultural Applied Technology Innovation Project of Shandong Province in 2018 (39797), Major Scientific and Technological Innovation Projects of Key R&D Program of Shandong Province (2019JZZY020607).

语种：英文

外文关键词：Ginger polysaccharide; Structure characterization; alpha-(1 -> 4)-glucan; Triple helix structure; Immunomodulatory activity

外文摘要：A neutral ginger polysaccharide fraction (NGP) was isolated from ginger, and its primary structures and immunomodulatory activity were investigated. The results showed that NGP had a low molecular weight of 6305 Da. NGP principally consisted of glucose (93.88%) together with minor levels of galactose (3.27%) and arabinose (1.67%). Besides, results of methylation analysis and 1D/2D NMR spectroscopy demonstrated that NGP was alpha-glucan which had the main chain of 1,4-linked alpha-D-Glcp and alpha-D-Glcp residues branched at C-6 position which was different from the common triple helical beta-glucans. NGP also displayed a remarkable immunological activity on the RAW264.7 cells in vitro. It could significantly enhance the proliferation of macrophages without cytotoxicity and increase the production of immune substances (NO, TNF-alpha, IL-1 beta and IL-6). The secretion at the concentration of 200 mu g/mL was 29.41 mu M, 1496.71, 44.30 and 1889.83 pg/mL for each substance, respectively. The results indicated that NGP could be a potential immune agent and might provide meaningful information for further chain conformation and immune mechanism research.