文献类型：期刊文献

英文题名：Fish Galectin8-Like Exerts Positive Regulation on Immune Response Against Bacterial Infection

作者：Niu, Jinzhong[1];Huang, Yu[1,2,3];Liu, Xinchao[1];Wu, Fenglei[1];Tang, Jufen[1,2,3];Wang, Bei[1,2,3];Lu, Yishan[1,2,3];Cai, Jia[1,2,3,4];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim,Coll Fishery, Guangdong Higher Educ Inst,Southern Marine Sci &, Zhanjiang, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;[3]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen, Peoples R China;[4]Guangxi Acad Sci, Guangxi Key Lab Marine Nat Prod & Combinat Biosyn, Guangxi Beibu Gulf Marine Res Ctr, Nanning, Peoples R China

年份：2020

卷号：11

外文期刊名：FRONTIERS IN IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000551842400001)、、Scopus(收录号：2-s2.0-85087756580)、WOS

基金：This work was supported by National Natural Science Foundation of China (grant nos. 31302226 and 31572651), National Key R&D Program of China (2018YFD0900501), Technology Planning Project of Guangdong Province of China (grant nos. 2015A020209181 and 2017A030307033), the Fund of Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW-2019-05), and Special foundation for achieving the first class of Guangdong Province (231419013).

语种：英文

外文关键词：galectin-8; Nile tilapia; monocytes; macrophages; Streptococcus agalactiae; immune response

外文摘要：Galectin-8 is a member of the galectin family that is involved in immune response against pathogens. However, the roles of fish galectin-8 during pathogen infection require comprehensive studies. In this study, a galectin-8 homolog (OnGal8-like, OnGal8-L) was characterized from Nile tilapia (Oreochromis niloticus), and its roles in response to bacterial infection were analyzed. TheOnGal8-Lcontains an open reading frame of 891 bp, encoding a peptide of 296 amino acids with two CRD regions of tandem-repeat galectin and two carbohydrate recognition sites. TheOnGal8-Lprotein shares 46.42% identities with reportedOreochromis niloticusgalectin-8 protein. Transcriptional expression analysis revealed that OnGal8-L was constitutively expressed in all examined tissues and was highly expressed in spleen. The transcript levels of OnGal8-L were up-regulated in the spleen, head kidney, and brain, followingStreptococcus agalactiae(S. agalactiae) challenge. Furtherin vitroanalysis indicated that the recombinant protein of OnGal8-L (rOnGal8L) could agglutinate erythrocyte,S. agalactiae, andA. hydrophilaand bindS. agalactiae, A. hydrophila, and various PAMPs (lipopolysaccharides, lipoteichoic acid, poly I:C, peptidoglycan, galactose, mannose, and maltose). Also, rOnGal8L could regulate inflammatory-related gene expression, phagocytosis, and a respiratory burst of monocytes/macrophages. Moreover,in vivoanalysis showed that OnGal8-L overexpression could protectO. niloticusfromS. agalactiaeinfection through modulating serum antibacterial activity (AKP, ACP, and LZM), antioxidant capacity (CAT, POD, and SOD), and monocyte/macrophage proliferation and cytokine expression, as well as reducing bacterial burden and decreasing tissue damage. Our results collectively indicate that OnGal8-L plays important regulatory roles in immune response against bacterial infection.