文献类型：期刊文献

中文题名：不同年龄高体鰤精巢转录组分析

英文题名：Comparative Transcriptome Analysis of Testis Development across Different Ages in Seriola dumerili

作者：林浩毅[1];邓秋霞[1];黄洋[1,2];茹笑影[2];杨佳慧[1];李广丽[1];朱春华[1,2]

机构：[1]广东海洋大学水产学院/广东省名特优鱼类生殖调控与繁育工程技术研究中心,广东湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),广东湛江524057

年份：2026

卷号：46

期号：1

起止页码：45

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：北大核心2023、、北大核心

基金：广东海洋大学自科项目(030301022301);湛江湾实验室人才团队引进项目(ZJW-2023-10);广东省现代化海洋牧场适养品种核心技术攻关项目(2024-MRB-00-001)。

语种：中文

中文关键词：高体鰤;不同年龄;精巢;转录组

外文关键词：Seriola dumerili;different ages;testis;transcriptome

中文摘要：【目的】探究人工养殖条件下,不同年龄阶段高体鰤(Seriola dumerili)精巢发育调控的分子机制。【方法】对1龄和2龄高体鰤精巢进行组织学观察、转录组测序分析、基因功能注释、差异基因筛选和鉴定,并利用实时荧光定量PCR检测高体鰤中精巢发育相关基因的表达。【结果】组织学分析表明,高体鰤1龄精巢为I期,2龄精巢为IV期。2个年龄阶段的高体鰤精巢转录组获得总干净读数为263476570,注释到21258个基因,其中1108个为差异表达基因。功能富集分析发现,大量差异基因参与了生殖相关的代谢通路,包括类固醇激素合成、GnRH信号通路、TGF-beta信号通路和Wnt信号通路等。转录组数据和定量结果表明,与1龄鱼相比,2龄鱼精巢中spata18、spata6、sycp3、smc3、sycp2、mns1、spag17、rsph1、spef2、spa17和spaca6等11个基因显著上调,gsdf、cyp19a1a、foxl2、gdf3和bmp8a等5个基因显著下调。【结论】本研究通过对1龄和2龄高体鰤精巢转录组测序分析,筛选出随年龄变化的精巢发育相关差异表达基因和代谢通路,从分子层面解析了高体鰤精巢发育的调控机制。

外文摘要：【Objective】This study aims to investigate the molecular mechanisms regulating testicular development in greater amberjack(Seriola dumerili)at different age stages under artificial aquaculture conditions.【Methods】Histological observation,transcriptome sequencing analysis,gene functional annotation,and screening and identification of differentially expressed genes(DEGs)were performed on the testis of 1-year-old and 2-year-old greater amberjack.The expression levels of genes related to testicular development were detected using real-time quantitative PCR(qPCR).【Results】Histological analysis showed that the testis of 1-year-old fish were at Stage I,while those of 2-year-old fish were at Stage IV.Transcriptome sequencing of testis from 1 and 2 age groups yielded 263476570 clean reads,corresponding to 21258 annotated genes,among which 1108 were identified as differentially expressed genes(DEGs).Functional enrichment analysis revealed that numerous DEGs were involved in reproduction-related metabolic pathways,including steroid hormone biosynthesis,GnRH signaling pathway,TGF-beta signaling pathway,and Wnt signaling pathway.Both transcriptome data and qPCR results indicated that,compared with 1-year-old fish,11 genes,namely spata18,spata6,sycp3,smc3,sycp2,mns1,spag17,rsph1,spef2,spa17,and spaca6 were significantly up-regulated in the testis of 2-year-old fish,while 5 genes,namely gsdf,gdf3,and foxl2,cyp19a1a,and bmp8 were significantly down-regulated.【Conclusion】Through transcriptome sequencing analysis of testis from 1-year-old and 2-year-old greater amberjack,this study identified age-related differentially expressed genes and metabolic pathways involved in testicular development,preliminarily revealing regulation mechanisms of testis development in S.dumerili at the molecular level.