文献类型：期刊文献

英文题名：Immune response and protective efficacy of two new adjuvants, MontanideTM ISA 763B VG and MontanideTM GEL02, administered with a Streptococcus agalactiae ghost vaccine in Nile tilapia (Oreochromis niloticus)

作者：Wangkaghart, Eakapol[1];Deville, Sebastien[2];Wang, Bei[3];Srisapoome, Prapansak[4];Wang, Tiehui[5];Secombes, Christopher J.[5]

机构：[1]Mahasarakham Univ, Fac Technol, Res Unit Excellence Trop Fisheries & Technol, Div Fisheries,Dept Agr Technol, Kantarawichai, Mahasarakham, Thailand;[2]SEPPIC, 50 Blvd Natl,CS 90020, F-92257 La Garenne Colombes, France;[3]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim, Guangdong Higher Educ Inst,Coll Fishery, Zhanjiang, Peoples R China;[4]Kasetsart Univ, Fac Fisheries, Dept Aquaculture, Bangkok 10900, Thailand;[5]Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen, Scotland

年份：2021

卷号：116

起止页码：19

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000725313100003)、、WOS

基金：This research was financially supported by the Office of the Permanent Secretary (OPS) and National Research Council of Thailand (NRCT), through the MRG Grant for the Development of New Lecturer (grant no. RGNS63-149) awarded to E.W. This Project was partially supported By Mahasarakham University. B.W. was supported by the National Natural Science Foundation of China (Grant No. 31702386), and International Cooperation Science & Technology Planning Project of Guangdong Province of China (Grant No. 2017A050501037).

语种：英文

外文关键词：Streptococcus agalactiae; Immune  response; New adjuvants; Montanide < sup > TM <; sup > ISA 763B VG; Montanide < sup > TM <; sup > GEL02; Ghost vaccine; Nile tilapia

外文摘要：Streptococcus agalactiae is one of the most important pathogens infecting tilapia worldwide and causes meningoencephalitis, septicemia and high mortalities with considerable losses. Various types of vaccines have been developed against S. agalactiae infection, such as inactivated vaccines, live attenuated vaccines and subunit vaccines. Bacterial ghosts (BGs) are nonliving, empty cell envelopes and have been reported as novel vaccine candidates. Therefore, the main aims of this study were to develop an S. agalactiae ghost vaccine (SAGV) and to evaluate the immune response and protective effect of SAGV against S. agalactiae with two novel adjuvants, MontanideTM ISA 763B VG and MontanideTM GEL02. Nile tilapia, mean weight 50 g, were divided into four groups as follows; 1) fish injected with PBS as control, 2) fish injected with the SAGV alone; 3) fish injected with the SAGV+MontanideTM ISA 763B VG; and 4) fish injected with SAGV+MontanideTM GEL02. Following vaccination, innate immunity parameters including serum lysozyme, myeloperoxidase, catalase, and bactericidal activity were all significantly enhanced. Moreover, specific serum IgM antibodies were induced and reached their highest level 2-8 weeks post vaccination. Importantly, the relative percent survival of tilapia vaccinated against the SAGV formulated with both adjuvants was 80-93%. Furthermore, the transcription of immune-related genes (IgM, TCR beta, IL-1 beta, IL-8 and TNF alpha) were up-regulated in tilapia after vaccination, indicating that both cellular and humoral immune responses were induced by these adjuvanted vaccines. In summary, MontanideTM ISA 763B VG and MontanideTM GEL02 can enhance immunoprotection induced by the SAGV vaccine against streptococcosis, demonstrating that both have value as potential adjuvants of fish vaccines.