文献类型：期刊文献

中文题名：槲皮素对杂交石斑鱼生长性能、抗氧化能力和肠道菌群的影响

英文题名：Effects of Quercetin on Growth Performance, Antioxidant Capacity and Intestinal Microflora of Hybrid Grouper (Epinephelus fuscoguttatus♀×Epinephelus polyphekadion♂)

作者：罗君[1];付伟杰[1];杨二军[1];黄建盛[1,2];谢瑞涛[3];陈刚[1,2]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),广东湛江524025;[3]广东恒兴饲料实业股份有限公司,广东湛江524022

年份：2022

卷号：42

期号：4

起止页码：13

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD、、北大核心、北大核心2020

基金：国家重点研发计划项目(2020YFD0900200);广东省科技计划项目(2016B020201009)。

语种：中文

中文关键词：槲皮素;杂交石斑鱼;褐点石斑鱼;清水石斑鱼;生长性能;抗氧化;NF-κB;肠道菌群

外文关键词：quercetin;hybrid grouper;Epinephelus fuscogutatus;Epinephelus polyphekadion;growth performance;antioxidant;NF-κB;intestinal microflora

中文摘要：【目的】探明饲料中添加槲皮素(quercetin,QE)对杂交石斑鱼(Epinephelus fuscoguttatus♀×Epinephelus polyphekadion♂)生长性能、肝肠抗氧化能力、肠道核因子-kappa B(Nuclear factor kappa B,NF-κB)通路相关基因表达及肠道菌群的影响。【方法】QE分别按照0(FM组)、0.80(QEL组)、1.60(QEM组)、3.20(QEH组)mmol/kg加入基础饲料,投喂体质量为(10.10±0.02)g的杂交石斑鱼幼鱼56 d,测定石斑鱼生长性能、肝肠抗氧化酶活性,用实时定量PCR分析肠道NF-κB通路相关基因表达量,用高通量测序技术分析肠道菌群结构。【结果】QEH组杂交石斑鱼增重率(WGR)、特定增长率(SGR)和终末体质量(FW)显著提高,存活率(SR)显著降低(P<0.05)。与FM组相比,QEM组肝脏超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)活性显著提高(P<0.05),QEH组肝脏GPX和过氧化氢酶(CAT)活性显著提高(P<0.05)。QE激活肠道NF-κB信号通路,上调肿瘤坏死因子(TNF-α)、TNF受体相关因子2(TRAF-2)、TNF受体超家族成员1A(TNFRSF1A)、干扰素-γ(IFN-γ)和核因子κBp65亚单位(NF-κBp65)表达水平,下调NF-κB抑制剂-α(IκB-α)的表达。与FM组相比,QEM和QEH组的运算分类单位(OTU)数目显著下降(P<0.05),ACE、Chao1、Simpson、Shannon指数无显著差异(P>0.05)。偏最小二乘判别分析显示,FM、QEL和QEH三组间β多样性指数存在显著差异(P<0.05)。QEM组Bifidobacterium显著增加(P<0.05)。【结论】饲料中添加0.8~3.2 mmol/kg QE可降低杂交石斑鱼的存活率,增加肝脏抗氧化能力;QE可激活肠道中NF-κB信号通路,降低肠道微生物多样性,QE有肠道促炎活性。

外文摘要：【Objective】 To investigate the effects of adding quercetin(QE) to the feed on the hybrid grouper(Epinephelus fuscoguttatus ♀ ×Epinephelus polyphekadion ♂) growth performance, liver and intestine antioxidant capacity, intestinal Nuclear factor kappa B(NF-κB) pathway related gene expression and intestinal flora. 【Method】The QE was added to the basal feed at the concentration of0(FM), 0.80(QEL), 1.60(QEM), 3.20(QEH) mmol/kg, and hybrid grouper juveniles weighed(10.10 ±0.02) g were fed for 56 days. The growth performance, liver and intestinal antioxidant enzyme activity indexes were measured, and intestinal NF-κB pathway-related gene expression of grouper were analyzed by Quantitative Real-time PCR. Analysis of intestinal flora structure by high-throughput sequencing technology. 【Result】 The weight gain rate(WGR), specific growth rate(SGR) and final body mass(FW) of hybrid grouper in the QEH group were significantly increased, survival rate(SR)was significantly decreased(P < 0.05). Compared with FM group, QEM group’s liver superoxide dismutase(SOD) and glutathione peroxidase(GPX) activities were significantly increased, and QEH group ’ s liver GPX and catalase(CAT) activities were significantly increased(P<0.05). QE activated the intestinal NF-κB signaling pathway and up-regulated tumor necrosis factor α(TNF-α), TNF receptor-associated factor 2(TRAF-2), TNF receptor superfamily member 1A(TNFRSF1A), interferon-γ(IFN-γ) and nuclear factor κBp65 subunit(NF-κBp65) expression levels, and down-regulated NF-κB inhibitor-α(IκB-α). Compared with FM group, the numbers of OTUs were significantly decreased in QEM and QEH groups(P<0.05), and there were no significant differences in ACE, Chao1, Simpson and Shannon indices(P>0.05). PLS-DA analysis showed significant beta diversity index differences between FM, QEL and QEH groups(P<0.05). Bifidobacterium was significantly increased in the QEM group(P<0.05). 【Conclusion】Within the scope of this experiment, the addition of 0.8-3.2 mmol/kg QE to the feed reduced the survival rate and increased the hepatic antioxidant capacity of hybrid grouper;in addition, QE had intestinal pro-inflammatory activity by activating the NF-κB signaling pathway in the intestine and reducing intestinal microbial diversity.