文献类型：期刊文献

英文题名：Metagenomic insight into the mechanisms of accelerated di-2-ethylhexyl phthalate (DEHP) degradation in vermicomposting

作者：Wei, Ting[1];Zhen, Zhen[1];Huang, Fengcheng[1];Li, Huijun[1];Wu, Weilong[1];Yang, Changhong[1];Yang, Guiqiong[1];Liang, Yan-Qiu[2];Lin, Zhong[2,3];Zhang, Dayi[4,5,6]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Res Inst, Shenzhen 518108, Peoples R China;[4]Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China;[5]Jilin Univ, Key Lab Groundwater Resources & Environm, Minist Educ, Changchun 130021, Peoples R China;[6]Shenyang Univ, Minist Educ, Key Lab Reg Environm & Ecorestorat, Shenyang 110044, Peoples R China

年份：2024

卷号：33

外文期刊名：ENVIRONMENTAL TECHNOLOGY & INNOVATION

收录：SCI-EXPANDED(收录号：WOS:001129939700001)、、EI(收录号：20234915162640)、Scopus(收录号：2-s2.0-85178586435)、WOS

基金：This work was financially supported by National Natural Science Foundation of China (41977125, 41907033) , Natural Science Foundation of Guangdong Province (2022A1515010867, 2022A1515010630, 2023A1515012225) , Shenzhen Fundamental Research Program (JCYJ20220818103609020) , Key Research and Development Program of Tibet Autonomous Region (XZ202001ZY0003G) and Open Fund from the Key Lab of Eco-restoration of Regional Contaminated Environment (Shenyang University) , Ministry of Education (KF-23-13) . DZ acknowledges support from Chinese Government's Thousand Talents Plan for Young Professionals.

语种：英文

外文关键词：Phthalate acid ester; Earthworm; Degradation pathway; Metagenomic; Bacterial community; Degradation genes

外文摘要：Earthworms can expedite di-(2-ethylhexyl) phthalate (DEHP) degradation in soils, but limited information is available on the key DEHP-degradation pathways and related genes during the vermicomposting process. In this study, DEHP degradation, degradation-related genes and bac-terial communities were investigated by metagenomic analysis. DEHP degradation efficiency was significantly and 65.69% higher in vermicomposting treatment than natural soils. Earthworm supplement remarkably increased the contents of humic acid, humus and fulvic acid in soils. Both humic acid and earthworm gut positively stimulated soil microbes potentially responsible for DEHP degradation. Betaprotebacteria, Acidobacteria, Variovorax, Hydrogenophaga, Limnobacter, Ramlibacter, Pseudomonas, Acinetobacter, Paracoccus and Achromobacter significantly contributed to DEHP degradation pathways. From functional gene analysis, there were remarkable differences in dominant DEHP degradation pathways between soils (catechol pathway), earthworm cast (protocatechuate pathway), and earthworm gut (protocatechuate and catechol pathways). Our findings proposed two possible mechanisms of earthworms in accelerating DEHP degradation, stimulating the activities of indigenous degraders to augment the catechol pathway in soils and providing an extra protocatechuate pathway in earthworm gut. This study, for the first time, offers new insights into the impacts of vermicomposting on DEHP degradation genes and path-ways, providing valuable scientific evidence for improving DEHP bioremediation in contaminated agricultural soils.