文献类型：期刊文献

中文题名：内生芽孢杆菌TB2防治辣椒疫病效果及其机理初探

英文题名：Biological control of Pepper Phytophthora blight by endophytic TB2 (Bacillus sp. )

作者：邱思鑫[1];何红[2];阮宏椿[3];关雄[3];胡方平[3]

机构：[1]福建农林大学植保学院;[2]湛江海洋大学农学院;[3]福建农林大学植保学院福建农林大学农药生物化学教育部重点实验室

年份：2004

卷号：34

期号：2

起止页码：173

中文期刊名：植物病理学报

外文期刊名：Acta Phytopathologica Sinica

收录：CSTPCD、、北大核心2000、CSCD2011_2012、北大核心、CSCD

基金：国家"八六三"项目(2002AA245011)资助;福建省科学技术厅项目(2002N004)资助;国家自然科学基金项目(30370968)资助

语种：中文

中文关键词：内生芽孢杆菌;TB2防治;辣椒疫病;作用机理

外文关键词：capsicum; Phytophthora blight; endophytic Bacillus; biological control

中文摘要：内生芽孢杆菌(Bacillus sp.)TB2对辣椒疫霉(Phytophthora capsici)引起的辣椒苗和果疫病有良好的防治效果,菌液浸种处理的苗接种病菌后20 d防病效果可达70.0％;菌液浇灌后48 h的苗接种病菌后20 d防病效果可达88.1％;菌液喷雾处理的果接种病菌后14 d防病效果可达65.2％。喷雾或浇灌菌株培养液后间隔24 h以上接种病原菌的防病效果比二者同时接种时高。生防菌菌体及其胞外分泌物均有防病作用,其胞外分泌物的速效性较好,菌体的持效性较好。菌株胞外分泌物对病菌菌丝生长、孢子囊形成和游动孢子释放均有明显的抑制作用。菌液可诱导植物抗性相关酶的变化,但其与病菌共同接种于辣椒时诱导作用可相互影响。疫霉菌处理可使辣椒果的丙二醛(MDA)含量和过氧化物酶(POD)、超氧化歧化酶(SOD)、过氧化氢酶(CAT)活性出现明显的峰值;菌液处理可诱导辣椒SOD和POD活性增强;但菌液与病菌共同处理后,辣椒果的MDA含量和SOD、POD、CAT的活性均较低,与清水对照接近,表现出一种相对稳定的状态。

外文摘要：Endophytic Bacillus sp. strain TB2 isolated from stem of tobacco was an efficient biological control agent to control Pepper Phytophthora blight. Seed coating and root drenching with TB2 cultures gave 70. 0% and 88. 1% control efficacies respectively, on capsicum seedlings 20 d after inoculation of the pathogen P. capsici, while spraying on fruits gave 65. 2% control efficacy to the fruit Phytophthora blight 14 d after inoculating the pathogen. The research on biological control mechanisms showed that the strain TB2 could inhibit the mycelial growth and reduce the sporangia and zoospores production of P. capsici. The treatment with strain TB2 considerably increased the SOD and POD activities in the capsicum fruits. Inoculation with P. capsici on fruit also significantly increased the MDA content and SOD, POD and CAT activities. However, the MDA content and SOD, POD and CAT activity of the capsicum fruits co-inoculated with the strain TB2 and P. capsici at the same time did not remarkably change comparing with fruits inoculating with sterile water.