文献类型：期刊文献

中文题名：马氏珠母贝酶解产物清除自由基活性的研究

英文题名：STUDY ON FREE RADICAL SCAVENGING ACTIVITY OF ENZYMATIC HYDROLYSATE OF PINCTADA MARTENSII EDIBLE PART

作者：曹文红[1];吴红棉[1];范秀萍[1];杜志伟[1];冯淑冰[1]

机构：[1]广东海洋大学食品科技学院

年份：2009

卷号：30

期号：1

起止页码：13

中文期刊名：食品研究与开发

外文期刊名：Food Research and Development

收录：CSTPCD、、北大核心2008、北大核心

基金：广东海洋大学自然科学团队项目(0612258)

语种：中文

中文关键词：马氏珠母贝;酶解;自由基清除活性;分子量分布

外文关键词：Pinctada martensii; enzymatic hydrolysis; free radicals scavenging activity; molecular weight distribution

中文摘要：利用胰酶、枯草杆菌中性蛋白酶,在50℃、pH为7.0～8.0、料水比1:3的条件下,对马氏珠母贝肉双酶水解4h制备得到酶解产物及残渣。利用Alcalase2.4L、胃蛋白酶、复合蛋白酶、木瓜蛋白酶对残渣进行再次酶解。结果表明:Alcalase2.4L、复合蛋白酶对残渣的酶解效果较好;残渣的复合蛋白酶酶解产物自由基清除活性较强;马氏珠母贝肉酶解产物清除自由基活性较强的组分分子量在1910u～944u之间,残渣复合蛋白酶酶解产物清除自由基活性较强的组分分子量在3472u～834u之间。

外文摘要：Hydrolysate with free radical scavenging activity was prepared from the edible part of Pinctada martensii by pancreatin and neutral protease, under the condition of 50 ℃, pH7.0-8.0, solid water ratio 1：3 and reaction time 4 h. The offal and the hydrolysate were collected. Alcalase 2.4 L, Pepsin, Protamex and Papain were chosen to hydrolyze the offal. The results showed that Alcalase 2.4 L and Protamex had a good capacity for hydrolyzing the offal. The hydrolysate of the offal by Protamex exerted a relatively strong radical scavenging activity. The fraction of the gel column chromatogram of Pinctada martensii hydrolysate with molecular weight ranged from 1 910 u to 944 u exerted the strongest radical scavenging activity. The fraction of the gel column chromatogram of Pinctada martensii offal hydrolysate with molecular weight ranged from 3 472 u to 834 u exerted the strongest radical scavenging activity.