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41 条 记 录,以下是 1-30

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A Sensitive and Validated Method for Determination of T-2 and HT-2 Toxin Residues in Shrimp Tissues by LC-MS/MS
FOOD ANALYTICAL METHODSLu, Pengli Wu, Chaojin Shi, Qi Wang, Yaling Sun, Lijun Liao, Jianmeng Zhong, Saiyi Xu, Defeng Chen, Jinjun Liu, Ying Li, Jianrong Gooneratne, Ravi  出版年:2016
Accurate analyses of T-2 and HT-2 toxin in aquatic organisms including shrimp are important as these two toxins are increasingly detected in aquatic cereal-based feed. Therefore, the potential for these toxins to enter t...
Production optimization, purification, and characterization of a novel acid protease from a fusant by Aspergillus oryzae and Aspergillus niger
EUROPEAN FOOD RESEARCH AND TECHNOLOGYLi, Caihong Xu, Defeng Zhao, Mouming Sun, Lijun Wang, Yaling  出版年:2014
The production of a novel acid protease was enhanced by 44 % through statistical optimization. The cultural parameters, such as inoculum size, temperature, moisture content, and incubation time, were 8.59 x 10(5) g(-1) d...
Inhibitory effect of glucose oxidase from Bacillus sp CAMT22370 on the quality deterioration of Pacific white shrimp during cold storage
LWT-FOOD SCIENCE AND TECHNOLOGYXu, Defeng Sun, Lijun Li, Caihong Wang, Yaling Ye, Riying  出版年:2018
The retarding effect of glucose oxidase (GOD) from Bacillus sp.CAMT22370 on the quality degradation of Litopenaeus vannwnEI was investigated in comparison with the commonly used preservatives of sodium sulfite (SS), phyt...
A sensitive method for simultaneous quantitative determination of surfactin and iturin by LC-MS/MS
ANALYTICAL AND BIOANALYTICAL CHEMISTRYDeng, Qi Wang, Wenjing Sun, Lijun Wang, Yaling Liao, Jianmeng Xu, Defeng Liu, Ying Ye, Riying Gooneratne, Ravi  出版年:2017
Surfactin and iturin are antimicrobial lipopeptides produced from Bacillus spp. and have significant prospective applications in many fields. Therefore, accurate analysis of these lipopeptides in the fermented product of...
Removal of aflatoxin b1 and t-2 toxin by bacteria isolated from commercially available probiotic dairy foods
FOOD SCIENCE AND TECHNOLOGY INTERNATIONALOndiek, Walter Wang, Yaling Sun, Lijun Zhou, Langhua On, Stephen L. W. Zheng, Haotian Ravi, Gooneratne  出版年:2022
This study isolated lactic acid bacteria from commercially available probiotic foods to determine thEIr capacity to remove aflatoxin B-1 (AFB(1)) and trichothecene-2 (T-2). The removal rates by original live and heat-tre...
环境条件对腐败希瓦氏菌生物被膜形成能力的影响
《中国食品学报》邓旗 孙力军 王雅玲 蒲月华 李振华 励建荣  出版年:2013
目的:研究环境条件对腐败希瓦氏菌生物被膜形成的影响。方法:采用改良的96微孔板法,通过改变环境因素,研究不同培养时间、菌体浓度和理化因子对腐败希瓦氏菌生物被膜形成能力的影响。用玻璃试管法和扫描电镜观察,以不锈钢片或聚乙烯...
关键词:希瓦氏菌  环境条件  生物被膜  特性  被引量:9
Antimicrobial peptide AMPNT-6 from Bacillus subtilis inhibits biofilm formation by Shewanella putrefaciens and disrupts its preformed biofilms on both abiotic and shrimp shell surfaces
FOOD RESEARCH INTERNATIONALDeng, Qi Pu, Yuehua Sun, Lijun Wang, Yaling Liu, Yang Wang, Rundong Liao, Jianmeng Xu, Defeng Liu, Ying Ye, Riying Fang, Zhijia Gooneratne, Ravi  出版年:2017
Shewanella putrefaciens biofilm formation is of great concern for the shrimp industry because it adheres easily to food and food-contact surfaces and is a source of persistent and unseen contamination that causes shrimp ...
对虾中T-2毒素的残留规律及其对雄性小鼠的遗传毒性效应
《现代食品科技》吴朝金 莫冰 王雅玲 黄展锐 何婉莹 苏静怡 徐德峰 孙力军  出版年:2015
研究了对虾中T-2毒素急性暴露的残留规律,并探明其对雄性小鼠的遗传毒性效应。实验以LC-MS/MS检测肌肉注射染毒后,对虾中T-2毒素残留量,并经灌胃,检测毒虾中T-2毒素对小鼠遗传毒性的危害。结果发现,对虾中游离态T-...
关键词:对虾  T-2毒素  雄性小鼠  精子畸形  微核  被引量:5
Preparation of T-2-glucoronide with Rat Hepatic Microsomes and Its Use along with T-2 for Activation of the JAK/STAT Signaling Pathway in RAW264.7 Cells
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRYWang, Xing Wang, Yaling Wang, Yapei Sun, Lijun Gooneratne, Ravi  出版年:2017
T-2 toxin (T-2), one of the most toxic trichothecene A-type mycotoxins, is biotransformed in animal tissues to modified T-2s (mT-2s) including T-2-glucuronide (T-2-GlcA). In this study, the optimal conditions for T-2-Glc...
T-2毒素对凡纳滨对虾的经口急性毒性效应研究
《现代食品科技》王雅玲 代喆 孙力军 吕鹏莉 徐德峰 刘颖 叶日英 聂芳红  出版年:2015
通过T-2毒素微胶囊毒饵料一次性染毒凡纳滨对虾,采用模型拟合法测定T-2毒素对凡纳滨对虾经口LD50,并分析Ca2+-ATPase、多酚氧化酶(PPO)活力以及肌肉病理组织学变化,进而探明T-2毒素对凡纳滨对虾急性毒性效...
关键词:T-2毒素  凡纳滨对虾  急性毒性  被引量:4
脂肽和茶多酚对副溶血弧菌的协同抑菌效应和机理
《食品科学》刘唤明 张文滔 吴燕燕 孙力军 王雅玲 刘颖 张雪梅 洪鹏志 吉宏武  出版年:2017
研究脂肽和茶多酚对副溶血弧菌的协同抑菌效应和机理,为脂肽协同茶多酚应用于副溶血弧菌的控制提供理论依据。利用"棋盘法"研究脂肽和茶多酚对副溶血弧菌的协同抑菌效应;通过研究脂肽和茶多酚独立作用及协同作用对细胞膜完整性、细胞蛋...
关键词:脂肽  茶多酚  副溶血弧菌  协同抑菌效应  协同抑菌机理  被引量:4
冷藏南美白对虾丝氨酸蛋白酶活力与黑变相关性研究
《现代食品科技》徐德峰 李彩虹 孙力军 王雅玲 叶日英 刘唤明 张永平 励建荣  出版年:2014
为探明冷藏对虾黑变规律,研究了南美白对虾在为期10 d的冷藏过程中黑变与丝氨酸蛋白酶(serine protease,SP)的活性变化及其相关性。结果表明,南美白对虾感官黑变呈现出先慢后快的特征,前2 d基本无变化,第3...
关键词:冷藏南美白对虾  黑变  丝氨酸蛋白酶活力  被引量:3
对虾中T-2毒素对小鼠免疫功能和血清生化指标的影响
《中国食品学报》吴朝金 王雅玲 孙力军 徐德峰 张永平 励建荣  出版年:2015
研究了对虾中T-2毒素对小鼠免疫学和血清学方面的毒性效应。分别用低、中、高剂量T-2毒素注射染毒对虾的不同组织灌胃小鼠1周,检测小鼠免疫功能指标(免疫器官指数、淋巴细胞转化能力、碳廓清能力、腹腔巨噬细胞吞噬能力)及血清生...
关键词:凡纳滨对虾  T-2毒素  小鼠  免疫功能  血清生化指标  被引量:3
高效液相色谱-串联质谱法测定3种水产品中的T-2毒素与HT-2毒素
《食品科学》王小博 施琦 王雅玲 廖建萌 刘阳 吴移山 高平 励建荣  出版年:2016
建立高效液相色谱-串联质谱定量快速检测罗非鱼、南美白对虾和黄金贝中的T-2毒素与HT-2毒素方法。以10 mL乙酸乙酯作为提取溶剂,振荡提取,无水硫酸钠除水,定量移取5 mL提取液氮气吹干后用1 mL含有0.1%甲酸的甲...
关键词:T-2毒素  HT-2毒素  高效液相色谱-串联质谱  被引量:2
低剂量T-2/HT-2毒素生物转化产物的遗传毒性研究
《现代食品科技》邱妹 陈海燕 孙力军 王雅玲 徐德峰 聂芳红 励建荣  出版年:2015
本文为了研究低剂量T-2和HT-2毒素经弯曲假单胞菌(Pseudomonas geniculata)生物转化至无游离态毒素检出时,降解产物是否具有遗传毒性。通过连续7 d给小鼠灌胃不同剂量T-2/HT-2毒素降解产物,检...
关键词:T-2毒素  生物转化产物  微核实验  精子致畸  被引量:2
Effects of T-2 toxin on histopathology, fatty acid and water distribution of shrimp (Litopenaeus vannamEI) muscle
Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural WastesBi, Siyuan Huang, Zhanrui Wang, Yaling Nie, Fanghong Wang, Xiaobo Sun, Lijun Zhu, Zhiqiang Gooneratne, Ravi  出版年:2019
T-2 toxin (T-2), one of the naturally occurring mycotoxins, often accumulates in aquatic animals from contaminated feed. Shrimp (n = 30 per group) were fed with different concentrations (0, 0.5, 1.5, 4.5 and 13.5 mg?kg?1...
Bacillus sp.CAMT22370源葡萄糖氧化酶对南美白对虾贮藏性能的影响
《食品科学》徐德峰 叶日英 李彩虹 孙力军 王雅玲 莫日坚 杨桥  出版年:2017
培养Bacillus sp.CAMT22370菌株并提取葡萄糖氧化酶制成质量分数0.1%的粗酶液,分别以亚硫酸钠、植酸和VC为对照,探讨所得葡萄糖氧化酶对南美白对虾冷藏过程中感官、质构、挥发性盐基氮含量、菌落总数等品质指...
关键词:Bacillus  sp.CAMT22370  南美白对虾  冷藏  葡萄糖氧化酶  被引量:2
纳豆芽孢杆菌NT-6固态发酵罗非鱼下脚料条件优化及产脂肽组分分析
《食品科学》张宁 孙力军 王雅玲 廖建萌 邓旗 刘颖 徐德峰  出版年:2016
利用纳豆芽孢杆菌(Bacillus natto)NT-6,以罗非鱼下脚料为主要基质,进行固态发酵生产抗菌营养复合型活性肽,以提高抗菌脂肽产量为目的,对培养基和发酵条件进行优化。在对产生的脂肽组分进行分析的基础上,采用高效...
关键词:纳豆芽孢杆菌  罗非鱼下脚料  固态发酵  响应面设计  抗菌脂肽  高效液相色谱-质谱  被引量:2
对虾肠道中产毒镰孢菌抑制菌的筛选与鉴定
《现代食品科技》叶日英 王雅玲 孙力军 徐德峰 蔡坤荣 薜奕红 陈妍贤 刘昆  出版年:2015
为了获得对产毒镰孢菌具有抑制效应的海洋细菌,从对虾肠道中分离细菌,并将分离得到的优势细菌与产T-2毒素的禾谷镰孢菌(Fusarium graminearum)(FG1207)进行固体对峙培养,具有抑菌圈的细菌菌落即为产毒...
关键词:对虾肠道细菌  禾谷镰孢菌  T-2毒素  抑制  被引量:1
对虾酚氧化酶微量测定体系的建立与评价
《中国食品学报》徐德峰 李彩虹 孙力军 王雅玲 叶日英 廖建萌  出版年:2015
快速跟踪对虾保藏过程中的酚氧化酶活力变化,对于改善对虾感官品质和提高产业经济价值具有重要意义。基于酶促反应动力学原理,通过考察反应时间及底物浓度对酶活的影响,建立微量测定体系,并在测定准确度、速度和试剂消耗方面与常量法作...
关键词:对虾  酚氧化酶  微量测定体系  被引量:1
南海海域动物肠道中抗菌活性乳酸菌的分离及多样性分析
《食品科学》焦冬冬 任文彬 赵鸭美 侯小琴 刘颖 孙力军 王雅玲  出版年:2017
以湛江南海海域常见的10种海洋动物为研究对象,采用倾注平板法分离肠道乳酸菌,以单核细胞增生李斯特氏菌(Listeria monocytogenes)等5株有害菌为指示菌,采用双层牛津杯扩散法测定其抗菌活性,通过建立16S...
关键词:海洋动物肠道  抗菌活性  乳酸菌  16SrRNA基因  多样性  被引量:1
冷藏凡纳滨对虾色差值与若干典型质量性状的灰色关联分析
《中国食品学报》叶日英 孙力军 王雅玲 徐德峰 吉宏武 何俊杰  出版年:2018
探索凡纳滨对虾在冷藏过程色差值ΔE*与一些典型质量性状的相关程度。将对虾置于4℃冷藏,每隔1d取出对虾,检测典型质量性状:细菌总数(CFU)、挥发盐基氮(TVB-N)含量、多酚氧化酶(PPO)活性、蒸煮损失率、肌肉pH值...
关键词:凡纳滨对虾  冷藏  色差值ΔE*  典型质量性状  灰色关联分析  被引量:1
典型冷链贮藏南美白对虾优势菌菌相变化与感官品质相关性分析
《中国食品学报》徐德峰 李彩虹 孙力军 王雅玲 叶日英 廖建萌  出版年:2015
将南美白对虾在典型冷链温度-23,0,5℃条件下贮藏,通过菌落计数与观察、革兰氏染色、生理生化试验对优势菌进行鉴定,结合感官评分和细菌总数变化规律,分析南美白对虾在典型冷链贮藏温度下优势菌菌相变化与感官品质的相关性。结果...
关键词:南美白对虾  冷链温度  菌相变化  感官品质  被引量:1
凡纳滨对虾腐败优势菌的分离鉴定及其群体感应信号分子测定
《食品科学》李灿 张煜琛 孙力军 王雅玲 梁美晶 胡汉桥 徐德峰 刘颖 叶日英  出版年:2016
从凡纳滨对虾中分离和鉴定腐败优势菌,并对其进行群体感应信号分子AHLs和AI-2的测定。通过细菌自动生化鉴定系统和16S r DNA序列鉴定优势腐败菌;利用生物检测菌根癌农杆菌(Agrobacterium tumefac...
关键词:凡纳滨对虾  优势腐败菌  群体感应  信号分子  被引量:1
对虾中隐蔽态T-2毒素残留对雄性小鼠生长发育的影响及其遗传毒性效应
《中国食品学报》王小博 邱妹 王雅玲 孙力军 徐德峰 刘颖 叶日英 励建荣  出版年:2018
为探明对虾中隐蔽态T-2染毒对小鼠生长发育及遗传方面的毒性效应,采用定期递增剂量法进行20 d蓄积染毒实验,以对虾的虾头和肌肉匀浆液灌胃小鼠,连续7 d,记录小鼠器官指数、精子致畸率、微核率,分析对虾中T-2及隐蔽态残留...
关键词:T-2毒素  隐蔽态  精子畸形率  微核率  被引量:0
Cascading Mechanism Triggering the Activation of Polyphenol Oxidase Zymogen in Shrimp Litopenaeus vannamEI After Postmortem and the Correlation with Melanosis Development
Food and Bioprocess TechnologyXu, Defeng Yang, Xianqing Wang, Yaling Sun, Lijun  出版年:2020
Melanization is the major deterioration in organoleptic quality of shrimp Litopenaeus vannamEI during cold storage and leads to dramatical reduction of commercial value. So far, the biochemical cascade mechanism triggeri...
Evaluation the effect of mycotoxins on shrimp (Litopenaeus vannamEI) muscle and thEIr limited exposure dose for preserving the shrimp quality
Journal of Food Processing and PreservationFang, Zhijia Zhou, Langhua Wang, Yaling Sun, Lijun Gooneratne, Ravi  出版年:2019
Mycotoxins widely contaminated the feed ingredients of shrimp, threatening the aquatic products, but thEIr adverse effects on quality is less known. In this study, the adverse effects of T-2 toxin (T-2), aflatoxin B (AFB...
The effects of removing aflatoxin B1 and T-2 toxin by lactic acid bacteria in high-salt fermented fish product medium under growth stress
LWTYe, Lin Wang, Yaling Sun, Lijun Fang, Zhijia Deng, Qi Huang, Yuying Zheng, Peishan Shi, Qi Liao, Jianmeng Zhao, Jian  出版年:2020
The objective of this study was to determine the effects of a high salt culture medium (fish sauce and salted fish) on the detoxification capacity of Lactobacillus acidophilus (L.a), L. bulgaricus (L.b), and L. casEI (L....
L-CystEIne hydrochloride inhibits Aspergillus flavus growth and AFB1 synthesis by disrupting cell structure and antioxidant system balance
Journal of Hazardous MaterialsQiu, Mei Qiu, Lihong Deng, Qi Fang, Zhijia Sun, Lijun Wang, Yaling Gooneratne, Ravi Zhao, Jian  出版年:2023
Aflatoxin B1 (AFB1) is the most potent known naturally occurring carcinogen and pose an immense threat to food safety and human health. L-CystEIne hydrochloride (L-CH) is a food additive often used as a fruit and vegetab...
The Comparison between Compound Preservatives and Sodium Metabisulfite Treatment on the Anti-melanosis Effect of Penaeus vannamEI under Ice-temperature
Journal of Chinese Institute of Food Science and TechnologyLü, Yanfang Cai, Luyun Li, Yingchang Li, Xuepeng Yang, Mingduo Li, Jianrong Wang, Yaling Chen, Huajian  出版年:2017
To previent shrimp darkening, overdose of sulfite is generally applied, whichintroduces excessive sulfur dioxide in shrimp products, thus harms human health. In this study, we treated shimps with a compound anti-melanosi...
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