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131 条 记 录,以下是 1-30

Purification and characterization of a novel bacteriocin CAMT2 produced by Bacillus amyloliquefaciens isolated from marine fish Epinephelus areolatus
FOOD CONTROLAn, Junying Zhu, Wenjuan Liu, Ying Zhang, Xuemei Sun, Lijun Hong, Pengzhi Wang, Yaling Xu, Chunhou Xu, Defeng Liu, Huanming  出版年:2015
A novel bacteriocin named CAMT2 was produced by strain ZJHD3-06 which was isolated from the marine fish Epinephelus areolatus and identified as Bacillus amyloliquefaciens, Bacteriocin CAMT2 inhibits important food spoila...
Inhibitory activity of a novel antibacterial peptide AMPNT-6 from Bacillus subtilis against Vibrio parahaemolyticus in shrimp
FOOD CONTROLXu, Defeng Wang, Yaling Sun, Lijun Liu, Huanming Li, Jianrong  出版年:2013
Vibrio parahaemolyticus is a major foodborne pathogen in marine products. Effective methods for reducing V. parahaemolyticus in seafood would reduce the likelihood of foodborne outbreaks of Vibrio. AMPNT-6 is a novel ant...
Effects of T-2 toxin on growth, immune function and hepatopancreas microstructure of shrimp (Litopenaeus vannamei)
AQUACULTUREQiu, Mei Wang, Yaling Wang, Xiaobo Sun, Lijun Ye, Riying Xu, Defeng Dai, Zhe Liu, Ying Bi, Siyuan Yao, Yanpo Gooneratne, Ravi  出版年:2016
T-2 toxin (T-2) is a trichothecene-A-type mycotoxin produced by the fungus Fusarium spp., which can contaminate animal feed. It is toxic to many living cells especially the hepatocytes and bone marrow cells. The effects ...
A sensitive and validated immunomagnetic-bead based enzyme-linked immunosorbent assay for analyzing total T-2 (free and modified) toxins in shrimp tissues
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETYDeng, Qi Qiu, Mei Wang, Yaling Lv, Pengli Wu, Chaojin Sun, Lijun Ye, Riying Xu, Defeng Liu, Ying Gooneratne, Ravi  出版年:2017
Accurate analyses of total T-2 (free and modified) in aquatic organisms including shrimp are important as the hazard caused by T-2 has been caught increasing attention. Therefore, acurate analysis of free T-2 especially ...
Regulation by walnut protein hydrolysate on the components and structural degradation of photoaged skin in SD rats
FOOD & FUNCTIONXu, Defeng Li, Dan Zhao, Zijian Wu, Jiaxin Zhao, Mouming  出版年:2019
Skin photoaging induced by consecutive exposure of skin to ultraviolet radiation is primarily responsible for skin aging and preparation of food-derived ingredients with anti-aging functions has been the hot topic worldw...
A Sensitive and Validated Method for Determination of T-2 and HT-2 Toxin Residues in Shrimp Tissues by LC-MS/MS
FOOD ANALYTICAL METHODSLu, Pengli Wu, Chaojin Shi, Qi Wang, Yaling Sun, Lijun Liao, Jianmeng Zhong, Saiyi Xu, Defeng Chen, Jinjun Liu, Ying Li, Jianrong Gooneratne, Ravi  出版年:2016
Accurate analyses of T-2 and HT-2 toxin in aquatic organisms including shrimp are important as these two toxins are increasingly detected in aquatic cereal-based feed. Therefore, the potential for these toxins to enter t...
NT-6抗菌脂肽对凡纳滨对虾生长性能及养殖源头弧菌数的影响
《广东农业科学》石广举 孙力军 王雅玲 刘唤明 徐德峰 聂芳红 刘颖 叶日英  出版年:2014
为了评价纳豆菌抗菌脂肽对凡纳滨对虾(Litopenaeus vannamei)生长的影响和抑制对虾养殖源头弧菌数的作用效果,在基础饲料中添加0(A0对照组)、50(A1组)、100(A2组)、200 mg/kg(A3组)...
关键词:抗菌脂肽  凡纳滨对虾  促生长  弧菌  存活率  被引量:19
Berberine Hydrochloride Impact on Physiological Processes and Modulation of Twist Levels in Nasopharyngeal Carcinoma CNE-1 Cells
ASIAN PACIFIC JOURNAL OF CANCER PREVENTIONLi, Cai-Hong Wu, Dong-Fang Ding, Hang Zhao, Yang Zhou, Ke-Yuan Xu, De-Feng  出版年:2014
Objective: The main purpose of this work was to investigate the effect of berberine hydrochloride (BH) on the proliferation, apoptosis, migration, and invasion of CNE-1 nasopharyngeal carcinoma cells. Our results shed li...
细菌革兰氏染色探究式实验教学的设计和实施效果分析
《微生物学通报》徐德峰 李彩虹 王雅玲 叶日英 孙力军  出版年:2013
细菌革兰氏染色效果受多种因素的影响,基于探究式教学法的理论原则,本文将固定方式和脱色时间两个关键因素设计成梯度对比实验,通过结果的直观差异性使教学效果显著提高。更为重要的是,学生在分组和组内实验探索过程中,通过内在好奇心...
关键词:革兰氏染色  探究式教学  效果分析  被引量:19
Regulation of Thermostable Direct Hemolysin and Biofilm Formation of Vibrio parahaemolyticus by Quorum-Sensing Genes luxM and luxS
CURRENT MICROBIOLOGYGuo, Muhan Fang, Zhijia Sun, Lijun Sun, Dongfang Wang, Yaling Li, Can Wang, Rundong Liu, Yang Hu, Hanqiao Liu, Ying Xu, Defeng Gooneratne, Ravi  出版年:2018
Vibrio parahaemolyticus is a seafood opportunistic pathogen. There are evidences suggesting that virulence skills, including hemolytic activity and biofilm formation, are regulated by the luxM/luxS-dependent quorum-sensi...
纳豆菌抗菌肽APNT-6对凡纳滨对虾的低温保鲜效果
《水产学报》王东 孙力军 王雅玲 刘唤明 徐德峰 邓楚津 杜焕妍 励建荣  出版年:2012
为了解决因凡纳滨对虾货架期短而滥用化学保鲜剂等问题,实验通过微生物发酵,柱状色谱纯化得到一种新型生物保鲜剂——纳豆菌抗菌肽APNT-6,采用牛津杯法测定了其对凡纳滨对虾腐败菌的抑菌效果,并用浸保鲜剂方式将抗菌肽应用于凡纳...
关键词:凡纳滨对虾  抗菌肽  腐败菌  货架期  被引量:18
Oleic Acid Alleviates Cadmium-Induced Oxidative Damage in Rat by Its Radicals Scavenging Activity
BIOLOGICAL TRACE ELEMENT RESEARCHWang, Jingwen Zhang, Yuanyuan Fang, Zhijia Sun, Lijun Wang, Yaling Liu, Ying Xu, Defeng Nie, Fanghong Gooneratne, Ravi  出版年:2019
Toxic heavy metal cadmium wildly pollutes the environment and threats the human health. Effective treatment of cadmium-induced toxicity and organ damage is an important issue. Cadmium causes organ damage through inducing...
抗菌豆豉发酵菌株的筛选及其脂肽组分鉴定和特性研究
《中国生物工程杂志》孙力军 王雅玲 刘唤明 徐德峰 张永平 聂芳红  出版年:2013
目的:从豆豉中分离广谱抗菌活性菌株,并鉴定其发酵产物中的抗菌成分。方法:采用生理生化实验结合16SrRNA序列测定法鉴定目标分离株,通过LC-MS和ESI/CID技术对其抗菌组分进行鉴定。结果:从8个豆豉样品中分离出7株...
关键词:豆豉  纳豆菌  抗菌活性  抗菌脂肽  被引量:17
Production optimization, purification, and characterization of a novel acid protease from a fusant by Aspergillus oryzae and Aspergillus niger
EUROPEAN FOOD RESEARCH AND TECHNOLOGYLi, Caihong Xu, Defeng Zhao, Mouming Sun, Lijun Wang, Yaling  出版年:2014
The production of a novel acid protease was enhanced by 44 % through statistical optimization. The cultural parameters, such as inoculum size, temperature, moisture content, and incubation time, were 8.59 x 10(5) g(-1) d...
Assessment on the improvement of soy sauce fermentation by Aspergillus oryzae HG76
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGYXu, Defeng Li, Caihong Zhao, Mouming Fengc, Yunzi Sun, Lijun Wang, Yaling  出版年:2013
To assess the improvement of Aspergillus oryzae HG76 on soy sauce fermentation, the items of biochemical compositions, molecular weight distribution (MWD), free amino acids (FAA) and volatile flavor compounds (VFC) profi...
Inhibitory effect of glucose oxidase from Bacillus sp CAMT22370 on the quality deterioration of Pacific white shrimp during cold storage
LWT-FOOD SCIENCE AND TECHNOLOGYXu, Defeng Sun, Lijun Li, Caihong Wang, Yaling Ye, Riying  出版年:2018
The retarding effect of glucose oxidase (GOD) from Bacillus sp.CAMT22370 on the quality degradation of Litopenaeus vannwnei was investigated in comparison with the commonly used preservatives of sodium sulfite (SS), phyt...
Cascading Mechanism Triggering the Activation of Polyphenol Oxidase Zymogen in Shrimp Litopenaeus vannamei After Postmortem and the Correlation with Melanosis Development
FOOD AND BIOPROCESS TECHNOLOGYXu, Defeng Yang, Xianqing Wang, Yaling Sun, Lijun  出版年:2020
Melanization is the major deterioration in organoleptic quality of shrimp Litopenaeus vannamei during cold storage and leads to dramatical reduction of commercial value. So far, the biochemical cascade mechanism triggeri...
纳豆芽孢杆菌NT-6抗菌脂肽对凡纳滨对虾生长性能及非特异性免疫指标的影响
《广东农业科学》石广举 孙力军 王雅玲 刘唤明 徐德峰 聂芳红 刘颖 叶日英  出版年:2014
为了评价纳豆菌抗菌脂肽对凡纳滨对虾(Litopenaeus vannamei)生长和非特异免疫能力的影响,在基础组饲料中分别添加0 mg/kg(A0组,对照组)、50 mg/kg(A1组)、100 mg/kg(A2组)、...
关键词:抗菌脂肽  凡纳滨对虾  生长  非特异性免疫  被引量:14
Modeling inhibitory activity of a novel antimicrobial peptide AMPNT-6 from Bacillus subtilis against Vibrio parahaemolyticus in shrimp under various environmental conditions
FOOD CONTROLPu, Yuehua Sun, Lijun Wang, Yaling Qi, Deng Chen, Danmin Liu, Huanming Xu, Defeng Deng, Chujing Li, Jianrong  出版年:2013
Vibrio parahaemolyticus is recognized as the leading cause of human gastroenteritis associated with the consumption of seafood. NT-6 antimicrobial peptide (AMPNT-6) that is a novel antimicrobial peptide secreted by Bacil...
A sensitive method for simultaneous quantitative determination of surfactin and iturin by LC-MS/MS
ANALYTICAL AND BIOANALYTICAL CHEMISTRYDeng, Qi Wang, Wenjing Sun, Lijun Wang, Yaling Liao, Jianmeng Xu, Defeng Liu, Ying Ye, Riying Gooneratne, Ravi  出版年:2017
Surfactin and iturin are antimicrobial lipopeptides produced from Bacillus spp. and have significant prospective applications in many fields. Therefore, accurate analysis of these lipopeptides in the fermented product of...
Complete Genome of Bacillus velezensis CMT-6 and Comparative Genome Analysis Reveals Lipopeptide Diversity
BIOCHEMICAL GENETICSDeng, Qi Wang, Rundong Sun, Dongfang Sun, Lijun Wang, Yaling Pu, Yuehua Fang, Zhijia Xu, Defeng Liu, Ying Ye, Riying Yin, Sanjun Xie, Sisi Gooneratne, Ravi  出版年:2020
The complete genome sequence of Bacillus velezensis type strain CMT-6 is presented for the first time. A comparative analysis between the genome sequences of CMT-6 with the genome of Bacillus amyloliquefaciens DSM7(T), B...
一种新型抗菌肽APNT-6的溶血性和急性毒性评价
《水产学报》孙力军 王雅玲 刘唤明 徐德峰 聂芳红 邹志飞 陈捷 励建荣  出版年:2012
研究了一种以抗菌脂肽类物质surfactin、iturin和fengycin为主要成分的新型抗菌肽APNT-6的体外溶血和小鼠口服急性毒性,为食品应用安全性提供初步评估。将抗菌肽作100倍稀释置于兔血琼脂平板上的牛津杯中...
关键词:抗菌肽  小鼠  溶血毒性  口服急性毒性  被引量:12
Cytotoxicity of T-2 and modified T-2 toxins: induction of JAK/STAT pathway in RAW264.7 cells by hepatopancreas and muscle extracts of shrimp fed with T-2 toxin
TOXICOLOGY RESEARCHWang, Xing Wang, Yaling Qiu, Mei Sun, Lijun Wang, Xiaobo Li, Caihong Xu, Defeng Gooneratne, Ravi  出版年:2017
T-2 can be biotransformed in animal tissues to modified T-2s (mT-2s). Food contaminated with T-2 and/or mT-2s is a hazard to both animals and humans, including the immune system. In this study, Litopenaeus vannamei were ...
Combined stress of acute cold exposure and waterless duration at low temperature induces mortality of shrimp Litopenaeus vannamei through injuring antioxidative and immunological response in hepatopancreas tissue
JOURNAL OF THERMAL BIOLOGYXu, Defeng Wu, Jiaxin Sun, Lijun Qin, Xiaoming Fan, Xiuping Zheng, Xiaoxian  出版年:2021
High mortality is a frequent occurrence during live transport of shrimp species and the biochemical mechanism remains unknown. This study aimed to explore the influence of combined stress of acute cold exposure (AC) and ...
纳豆菌脂肽对金黄色葡萄球菌抑菌机理的研究
《食品工业科技》刘唤明 孙力军 王雅玲 徐德峰 刘阳 陈宏 唐晓山  出版年:2012
【目的】研究纳豆菌脂肽对金黄色葡萄球菌的抑菌机理。【方法】通过对金黄色葡萄球菌生长曲线、细胞膜通透性、细胞蛋白的合成、细胞超微结构、细胞代谢的影响等方面的研究,探讨纳豆菌脂肽对金黄色葡萄球菌的抑菌机理。【结果】脂肽可以使...
关键词:纳豆菌  脂肽  金黄色葡萄球菌  抑菌机理  被引量:11
食品微生物学课堂教学改革与实践
《现代农业科技》刘唤明 雷晓凌 刘颖 王玲 曾少葵 徐德峰 伍彬 叶日英  出版年:2016
结合食品微生物学课程的课堂教学实践,介绍了从教学内容、教学方法和教学手段这3个方面进行改革,取得了较好的教学效果。
关键词:食品微生物学  课堂教学  教学改革  被引量:11
纳豆菌抗菌脂肽对副溶血弧菌的抑菌机理
《食品科学》刘唤明 孙力军 王雅玲 徐德峰 刘阳 陈宏 唐晓山  出版年:2012
目的:对纳豆菌抗菌脂肽抑制副溶血弧菌的抑菌机理进行探讨。方法:通过纳豆菌抗菌脂肽对副溶血弧菌生长曲线、细胞超微结构、细胞膜通透性、细胞DNA的作用、细胞蛋白的合成、细胞代谢的影响等方面的研究,探讨纳豆菌抗菌脂肽抑制副溶血...
关键词:抗菌脂肽  纳豆菌  副溶血弧菌  抑菌机理  被引量:10
Biotransformation enzyme activities and phase I metabolites analysis in Litopenaeus vannamei following intramuscular administration of T-2 toxin
DRUG AND CHEMICAL TOXICOLOGYDeng, Yijia Wang, Yaling Sun, Lijun Lu, Pengli Wang, Rundong Ye, Lin Xu, Defeng Ye, Riying Liu, Ying Bi, Siyuan Gooneratne, Ravi  出版年:2018
T-2 toxin (T-2) is a type-A trichothecene produced by Fusarium that causes toxicity to animals. T-2 contamination of grain-based aquatic feed is a concern for the industries related to edible aquatic crustacean species s...
湛江对虾产业核心竞争力的构建及其可持续发展
《广东农业科学》宋广智 徐德峰 王雅玲 孙力军  出版年:2011
从产业经济和产业技术的角度梳理和剖析湛江对虾产业在养殖、加工和贸易流通环节所存在的主要问题,并提出相应策略。在产业技术方面,以资源保护、质量安全为基调,以"产、学、研"为行业科技研发机制,强调各环节的技术互助和协同,形成...
关键词:湛江  对虾产业  核心竞争力  可持续发展  被引量:9
Pathogenicity of Vibrio parahaemolyticus in Different Food Matrices
JOURNAL OF FOOD PROTECTIONWang, Rundong Sun, Lijun Wang, Yaling Deng, Yijia Liu, Ying Xu, Defeng Liu, Huanming Ye, Riying Gooneratne, Ravi  出版年:2016
The pathogenicity and virulence factors of Vibrio parahaemolyticus in four food matrices-shrimp, freshwater fish, pork, and egg-fried rice-were compared by measuring the thermostable direct hemolysin activity and total h...
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