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Effects of a commercial probiotic BS containing <i>Bacillus subtilis</i> and <i>Bacillus licheniformis</i> on growth, immune response and disease resistance in Nile tilapia, <i>Oreochromis niloticus</i>
FISH & SHELLFISH IMMUNOLOGYAbarike, Emmanuel Delwin Cai, Jia Lu, Yishan Yu, Huang Chen, Lihua Jian, Jichang Tang, Jufen Jun, Liang Kuebutornye, Felix K. A.  出版年:2018
The present study evaluated a commercial probiotic designated as BS (a mix of B. subtilis and B. licheniformis) to ascertain its efficacy and the dose necessary to improve growth, immune response, and disease resistance ...
Immunostimulatory effects of artificial feed supplemented with a Chinese herbal mixture on <i>Oreochromis niloticus</i> against <i>Aeromonas hydrophila</i>
FISH & SHELLFISH IMMUNOLOGYTang, Jufen Cai, Jia Liu, Ran Wang, Jiamin Lu, Yishan Wu, Zaohe Jian, Jichang  出版年:2014
The effects of a Chinese herbal mixture (CHM) composed of astragalus, angelica, hawthorn, Licorice root and honeysuckle on immune responses and disease resistant of Nile tilapia (Oreochromis niloticus GIFT strain) were i...
Influence of traditional Chinese medicine and <i>Bacillus</i> species (TCMBS) on growth, immune response and disease resistance in Nile tilapia, <i>Oreochromis niloticus</i>
AQUACULTURE RESEARCHAbarike, Emmanuel Delwin Jian, Jichang Tang, Jufen Cai, Jia Yu, Huang Chen Lihua Jun, Liang  出版年:2018
Multispecies herbs and probiotic bacteria offered to fish might enhance their immune response and increase disease resistance, but the dose effects of herbal-probiotic application remain unclear. Therefore, the effect of...
In vivo assessment of the probiotic potentials of three host -associated Bacillus species on growth performance, health status and disease resistance of Oreochromis niloticus against Streptococcus agalactiae
AQUACULTUREKuebutornye, Felix K. A. Tang, Jufen Cai, Jia Yu, Huang Wang, Zhiwen Abarike, Emmanuel Delwin Lu, Yishan Li, Yuan Afriyie, Gyamfua  出版年:2020
Four weeks feeding trial was conducted to assess the in vivo probiotic potentials of three host-associated Bacillus species in O. niloticus. The fish were divided into five treatment groups and fed with diets containing ...
Single-cell RNA-seq reveals different subsets of non-specific cytotoxic cells in teleost
GENOMICSNiu, Jinzhong Huang, Yu Liu, Xinchao Zhang, Zhiqiang Tang, Jufen Wang, Bei Lu, Yishan Cai, Jia Jian, Jichang  出版年:2020
Non-specific cytotoxic cells (NCC) are important cytotoxic leukocytes in teleost immune system. However, the NCC subsets have not been clarified. Thus, we create a comprehensive cell map of similar to 24,062 head kidney-...
NK-lysin from <i>Oreochromis niloticus</i> improves antimicrobial defence against bacterial pathogens
FISH & SHELLFISH IMMUNOLOGYHuang, Yu Zheng, Qi Niu, Jinzhong Tang, Jufen Wang, Bei Abarike, Emmanuel Delwin Lu, Yishan Cai, Jia Jian, Jichang  出版年:2018
NK-lysin, an effector of cytotoxic T cells and natural killer cells, is a potent antimicrobial peptide widely distributed in mammals. Homologues of NK-lysin have been discovered in several teleost species, but only sever...
Molecular characterization and expression of CD2BP2 in Nile tilapia (<i>Oreochromis niloticus</i>) in response to <i>Streptococcus agalactiae</i> stimulus
GENEGan, Zhen Wang, Bei Lu, Yishan Cai, Shuanghu Cai, Jia Jian, JiChang Wu, Zaohe  出版年:2014
CD2BP2 (CD2 cytoplasmic tail binding protein 2), one of several proteins interacting with the cytoplasmic tail of CD2, plays a crucial role in CD2-triggered T cell activation and nuclear splicing. The studies on CD2BP2 h...
Establishment of a new cell line from the snout tissue of golden pompano <i>Trachinotus ovatus</i>, and its application in virus susceptibility
JOURNAL OF FISH BIOLOGYYu, Y. Wei, S. Wang, Z. Huang, X. Huang, Y. Cai, J. Li, C. Qin, Q.  出版年:2016
A new marine-fish cell line, designated GPS, was established from the snout tissue of golden pompano Trachinotus ovatus. GPS cells multiplied well in Leibovitz's L-15 containing 10% foetal bovine serum (FBS) at 28 degree...
Short term starvation and re-feeding in Nile tilapia (<i>Oreochromis niloticus</i>, Linnaeus 1758): Growth measurements, and immune responses
AQUACULTURE REPORTSSakyi, Michael Essien Cai, Jia Tang, Jufen Xia, Liqun Li, Pengfei Abarike, Emmanuel Delwin Kuebutornye, Felix Kofi Agbeko Jian, Jichang  出版年:2020
The effects of short term starvation and re-feeding to satiation on compensatory growth performance, haematological parameters, biochemical parameters, and immune responses were investigated in Oreochromis niloticus of a...
Fish Galectin8-Like Exerts Positive Regulation on Immune Response Against Bacterial Infection
FRONTIERS IN IMMUNOLOGYNiu, Jinzhong Huang, Yu Liu, Xinchao Wu, Fenglei Tang, Jufen Wang, Bei Lu, Yishan Cai, Jia Jian, Jichang  出版年:2020
Galectin-8 is a member of the galectin family that is involved in immune response against pathogens. However, the roles of fish galectin-8 during pathogen infection require comprehensive studies. In this study, a galecti...
Conservation of structural and interactional features of CD28 and CD80/86 molecules from Nile tilapia (<i>Oreochromis niloticus</i>)
FISH & SHELLFISH IMMUNOLOGYHuang, Yu Wang, Zhiwen Zheng, Qi Tang, Jufen Cai, Jia Lu, Yishan Jian, Jichang  出版年:2018
Interaction of CD28 with CD80 or CD86 molecules provides a costimulatory signals required in T cell activation. In this study, we cloned and analyzed a CD28 gene (On-CD28) and a CD80/86 gene (On-CD80/86) from Nile tilapi...
Establishment and characterization of a novel cell line from the brain of golden pompano (<i>Trachinotus ovatus</i>)
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMALLi, Pengfei Zhou, Lingli Ni, Songwei Xu, Meng Yu, Yepin Cai, Jia Wei, Shina Qin, Qiwei  出版年:2016
Golden pompano is a commercially important marine fish that is widely cultured in China, Japan, and Southeast Asian countries but has been seriously threatened by pathogen. A novel cell line (TOGB) derived from the brain...
Development of DNA vaccines encoding ribosomal proteins (RplL and RpsA) against <i>Nocardia seriolae</i> infection in fish
FISH & SHELLFISH IMMUNOLOGYChen, Jianlin Chen, Zhenwei Wang, Wenji Hou, Suying Cai, Jia Xia, Liqun Lu, Yishan  出版年:2020
Nocardia seriolae, a Gram-positive pathogen, has been identified as the causative agent of fish nocardiosis. DNA vaccination has been proven to be effective in conferring protection against bacterial infection in fish. T...
A mixture of Chinese herbs and a commercial probiotic <i>Bacillus</i> species improves hemato-immunological, stress, and antioxidant parameters, and expression of HSP70 and HIF-1α mRNA to hypoxia, cold, and heat stress in Nile tilapia, <i>Oreochromis niloticus</i>
AQUACULTURE REPORTSAbarike, Emmanuel Delwin Jian, Jichang Tang, Jufen Cai, Jia Sakyi, Essien M. Kuebutornye, Felix K. A.  出版年:2020
A 56-day feeding trial was performed to evaluate the effect of herbal-probiotic supplements comprising a mixture of Chinese herbs (CH) (i.e., Astragalus membranaceus, Angelica sinensis, and Crataegus hupehensis) and or c...
Molecular cloning and characterization of a new G-type lysozyme gene (Ec-lysG) in orange-spotted grouper, <i>Epinephelus coioides</i>
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGYWei, Shina Huang, Youhua Huang, Xiaohong Cai, Jia Wei, Jingguang Li, Pengfei Ouyang, Zhengliang Qin, Qiwei  出版年:2014
Lysozyme acts as an innate immunity molecule against pathogen infection. In this study, a new G-type lysozyme gene with a typical G-type lysozyme domain (designated as Ec-lysG) was cloned and characterized from the orang...
VscO, a putative T3SS chaperone escort of <i>Vibrio alginolyticus</i>, contributes to virulence in fish and is a target for vaccine development
FISH & SHELLFISH IMMUNOLOGYZhou, Zejun Pang, Huanying Ding, Yu Cai, Jia Huang, Yucong Jian, Jichang Wu, Zaohe  出版年:2013
Type III secretion system (T3SS) in Vibrio alginolyticus is essential for its pathogenesis. VscO's homologous proteins FliJ, InvI and YscO have been suggested to be putative chaperone escorts although its function in V. ...
Traditional Chinese Medicine Enhances Growth, Immune Response, and Resistance to <i>Streptococcus agalactiae</i> in Nile Tilapia
JOURNAL OF AQUATIC ANIMAL HEALTHAbarike, Emmanuel Delwin Jian, Jichang Tang, Jufen Cai, Jia Yu, Huang Chen, Lihua  出版年:2019
Herbal supplements are suitable for improving fish health and combating diseases in fish culture. However, the mechanism of action of many herbal mixtures remains unclear. This study was conducted to evaluate the effects...
Influences of immunostimulants on phagocytes in cultured fish: a mini review
REVIEWS IN AQUACULTUREAbarike, Emmanuel D. Kuebutornye, Felix K. A. Jian, Jichang Tang, Jufen Lu, Yishan Cai, Jia  出版年:2019
Lower vertebrates like fish mostly depend on innate immunity as the first line of defence against invading pathogens. One of the defence mechanisms in innate immunity for fish is the use of phagocytic cells such as monoc...
Identification and characterization of tumor necrosis factor receptor (TNFR)-associated factor 3 from humphead snapper, <i>Lutjanus sanguineus</i>
FISH & SHELLFISH IMMUNOLOGYCai, Jia Xia, Hongli Huang, Yucong Tang, Jufen Jian, Jichang Wu, Zaohe Lu, Yishan  出版年:2015
Tumor necrosis factor receptor (TNFR)-associated factor 3(TRAF3) is a key regulator in TNFR and Toll-like receptor (TLRs)/RIG-I-like receptors (RLRs) signal pathway. Here, a TRAF3 gene (Ls-TRAF3, GenBank Accession No: Kj...
Effect of <i>Bacillus</i> Probiotics on the Immunological Responses of Nile Tilapia (<i>Oreochromis niloticus</i>): A Review
FISHESShija, Vicent Michael Amoah, Kwaku Cai, Jia  出版年:2023
The use of antibiotics and therapeutics for Nile tilapia has increased along with its increased production and intensification. Probiotics have been studied as an alternative to the excessive use of antibiotics in fish d...
Effects of starvation and subsequent re -feeding on intestinal microbiota, and metabolic responses in Nile tilapia, Oreochromis niloticus
AQUACULTURE REPORTSSakyi, Michael Essien Cai, Jia Tang, Jufen Abarike, Emmanuel Delwin Xia, Liqun Li, Pengfei Kuebutornye, Felix Kofi Agbeko Zou, Zihong Liang, Zhenyu Jian, Jichang  出版年:2020
The effects of starvation and re -feeding on Nile tilapia ( Oreochromis niloticus ) remain incompletely understood so far. In this study, the changes of intestinal microbiota, the metabolic and immune (antioxidant) respo...
Characterization of a tandem-repeat galectin-9 from Nile tilapia (<i>Oreochromis niloticus</i>) involved in the immune response against bacterial infection
FISH & SHELLFISH IMMUNOLOGYNiu, Jinzhong Huang, Yu Li, Yuan Wang, Zhiwen Tang, Jufen Wang, Bei Lu, Yishan Cai, Jia Jian, Jichang  出版年:2019
Galectin-9 is a < beta > -galactoside-binding lectin which could modulate a variety of biological functions including recognition, aggregation and clearance of pathogen. In this study, a galectin-9 homologue (OnGal-9) wa...
Comparison of protective efficacy between two DNA vaccines encoding DnaK and GroEL against fish nocardiosis
FISH & SHELLFISH IMMUNOLOGYChen, Jianlin Wang, Wenji Hou, Suying Fu, Weixuan Cai, Jia Xia, Liqun Lu, Yishan  出版年:2019
Fish nocardiosis is a chronic granulomatous bacterial disease mainly caused by three pathogenic bacteria, including Nocardia seriolae, N. asteroids and N. salmonicida. Molecular chaperone DnaK and GroEL were identified t...
Establishment and characterization of a cell line from the head kidney of golden pompano <i>Trachinotus ovatus</i> and its application in toxicology and virus susceptibility
JOURNAL OF FISH BIOLOGYLi, P. Zhou, L. Wei, S. Yang, M. Ni, S. Yu, Y. Cai, J. Qin, Q.  出版年:2017
A cell line derived from the head kidney of golden pompano Trachinotus ovatus (TOHK) was established and characterized in this study. The TOHK cells grew most rapidly at 28 degrees C and the optimum foetal bovine serum c...
Characterization of LPS-induced TNFα factor (LITAF) from orange-spotted grouper, <i>Epinephelus coioides</i>
FISH & SHELLFISH IMMUNOLOGYCai, Jia Huang, Youhua Wei, Shina Ouyang, Zhengliang Huang, Xiaohong Qin, Qiwei  出版年:2013
Lipopolysaccharide-induced TNF alpha factor (LITAF) is an important transcription factor that mediates cell apoptosis and inflammatory response. In the present study, we cloned and characterized a LITAF gene from orange-...
Starvation and re-feeding influence the growth, immune response, and intestinal microbiota of Nile tilapia (Oreochromis niloticus; Linnaeus 1758)
AQUACULTURESakyi, Michael Essien Cai, Jia Ampofo-Yeboah, Akwasi Anokyewaa, Melody Abena Wang, Zhiwen Jian, Jichang  出版年:2021
In the present study, the impacts of starvation and re-feeding on growth, immune response, and intestinal microbiota in Nile tilapia were evaluated. During starvation, the growth performance parameters including body wei...
Characterization of Galectin-2 from Nile tilapia (<i>Oreochromis niloticus</i>) involved in the immune response to bacterial infection
FISH & SHELLFISH IMMUNOLOGYNiu, Jinzhong Huang, Yu Niu, Jimin Wang, Zhiwen Tang, Jufen Wang, Bei Lu, Yishan Cai, Jia Jian, Jichang  出版年:2019
galectin-2 plays important roles in innate and adaptive immunity. In this study, galectin-2 (OnGal 2) was identified from Nile tilapia (Oreochromis niloticus). Its tissue distribution and expression patterns following ba...
Functional characterization of galectin-3 from Nile tilapia (<i>Oreochromis niloticus</i>) and its regulatory role on monocytes/macrophages
FISH & SHELLFISH IMMUNOLOGYNiu, Jinzhong Huang, Yu Liu, Xinchao Luo, Guoling Tang, Jufen Wang, Bei Lu, Yishan Cai, Jia Jian, Jichang  出版年:2019
Galectin-3 is a kind of beta-galactoside-binding lectin involved in host defense against pathogen infection. However, the immune functions of fish galectin-3 remain poorly understood. In this study, the roles of a fish g...
Identification of a mitochondrial-targeting secretory protein from <i>Nocardia seriolae</i> which induces apoptosis in fathead minnow cells
JOURNAL OF FISH DISEASESChen, Jianlin Xia, Liqun Wang, Wenji Wang, Zhiwen Hou, Suying Xie, Caixia Cai, Jia Lu, Yishan  出版年:2019
Nocardia seriolae is the main pathogen responsible for fish nocardiosis. A mitochondrial-targeting secretory protein (MTSP) 3141 with an N-terminal transit peptide (TP) from N. seriolae was predicted by bioinformatic ana...
Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of <i>Nocardia salmonicida</i>, the Causative Agent of Nocardiosis in Fish
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGYXia, Liqun Zhang, Honglian Lu, Yishan Cai, Jia Wang, Bei Jian, Jichang  出版年:2015
Nocardia salmonicida is one of the main pathogens of fish nocardiosis. The purpose of this study was to build a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of N. salmonicida...
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